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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Francisella tularensis subsp. holarctica Chaperone protein htpG (htpG), partial CSB-YP414473FDV
CSB-EP414473FDV
CSB-BP414473FDV
CSB-MP414473FDV
CSB-EP414473FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Ribonuclease PH (rph) CSB-YP414474FDV
CSB-EP414474FDV
CSB-BP414474FDV
CSB-MP414474FDV
CSB-EP414474FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP414475FDV
CSB-EP414475FDV
CSB-BP414475FDV
CSB-MP414475FDV
CSB-EP414475FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Lipid-A-disaccharide synthase (lpxB) CSB-YP414476FDV
CSB-EP414476FDV
CSB-BP414476FDV
CSB-MP414476FDV
CSB-EP414476FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Pantothenate synthetase (panC) CSB-YP414477FDV
CSB-EP414477FDV
CSB-BP414477FDV
CSB-MP414477FDV
CSB-EP414477FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Ribose-5-phosphate isomerase A (rpiA) CSB-YP414478FDV
CSB-EP414478FDV
CSB-BP414478FDV
CSB-MP414478FDV
CSB-EP414478FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP414479FDV
CSB-EP414479FDV
CSB-BP414479FDV
CSB-MP414479FDV
CSB-EP414479FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP414480FDV
CSB-EP414480FDV
CSB-BP414480FDV
CSB-MP414480FDV
CSB-EP414480FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Putative 3-methyladenine DNA glycosylase (FTA_0989) CSB-YP414481FDV
CSB-EP414481FDV
CSB-BP414481FDV
CSB-MP414481FDV
CSB-EP414481FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica 30S ribosomal protein S18 (rpsR) CSB-YP414482FDV
CSB-EP414482FDV
CSB-BP414482FDV
CSB-MP414482FDV
CSB-EP414482FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP414483FDV
CSB-EP414483FDV
CSB-BP414483FDV
CSB-MP414483FDV
CSB-EP414483FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica 50S ribosomal protein L13 (rplM) CSB-YP414484FDV
CSB-EP414484FDV
CSB-BP414484FDV
CSB-MP414484FDV
CSB-EP414484FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Translation initiation factor IF-1 (infA) CSB-YP414485FDV
CSB-EP414485FDV
CSB-BP414485FDV
CSB-MP414485FDV
CSB-EP414485FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Threonine--tRNA ligase (thrS), partial CSB-YP414486FDV
CSB-EP414486FDV
CSB-BP414486FDV
CSB-MP414486FDV
CSB-EP414486FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Transcription elongation factor GreA (greA) CSB-YP414487FDV
CSB-EP414487FDV
CSB-BP414487FDV
CSB-MP414487FDV
CSB-EP414487FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP414488FDV
CSB-EP414488FDV
CSB-BP414488FDV
CSB-MP414488FDV
CSB-EP414488FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Probable Fe (2+)-trafficking protein CSB-YP414489FDV
CSB-EP414489FDV
CSB-BP414489FDV
CSB-MP414489FDV
CSB-EP414489FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Cysteine--tRNA ligase (cysS) CSB-YP414490FDV
CSB-EP414490FDV
CSB-BP414490FDV
CSB-MP414490FDV
CSB-EP414490FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica tRNA (guanine-N (1)-)-methyltransferase CSB-YP414491FDV
CSB-EP414491FDV
CSB-BP414491FDV
CSB-MP414491FDV
CSB-EP414491FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica 50S ribosomal protein L10 (rplJ) CSB-YP414492FDV
CSB-EP414492FDV
CSB-BP414492FDV
CSB-MP414492FDV
CSB-EP414492FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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