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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Francisella tularensis subsp. holarctica Bifunctional protein FolD (folD) CSB-YP416513FDV
CSB-EP416513FDV
CSB-BP416513FDV
CSB-MP416513FDV
CSB-EP416513FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica 50S ribosomal protein L28 (rpmB) CSB-YP416514FDV
CSB-EP416514FDV
CSB-BP416514FDV
CSB-MP416514FDV
CSB-EP416514FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica DNA-directed RNA polymerase subunit alpha 2 (rpoA2) CSB-YP416515FDV
CSB-EP416515FDV
CSB-BP416515FDV
CSB-MP416515FDV
CSB-EP416515FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica tRNA (Ile)-lysidine synthase CSB-YP416516FDV
CSB-EP416516FDV
CSB-BP416516FDV
CSB-MP416516FDV
CSB-EP416516FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica SsrA-binding protein (smpB) CSB-YP416517FDV
CSB-EP416517FDV
CSB-BP416517FDV
CSB-MP416517FDV
CSB-EP416517FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Protein hfq (hfq) CSB-YP416518FDV
CSB-EP416518FDV
CSB-BP416518FDV
CSB-MP416518FDV
CSB-EP416518FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica DNA polymerase IV (dinB) CSB-YP416519FDV
CSB-EP416519FDV
CSB-BP416519FDV
CSB-MP416519FDV
CSB-EP416519FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Octanoyltransferase (lipB) CSB-YP416520FDV
CSB-EP416520FDV
CSB-BP416520FDV
CSB-MP416520FDV
CSB-EP416520FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Phosphoglycerate kinase (pgk) CSB-YP416521FDV
CSB-EP416521FDV
CSB-BP416521FDV
CSB-MP416521FDV
CSB-EP416521FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Leucine--tRNA ligase (leuS), partial CSB-YP416522FDV
CSB-EP416522FDV
CSB-BP416522FDV
CSB-MP416522FDV
CSB-EP416522FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP416523FDV
CSB-EP416523FDV
CSB-BP416523FDV
CSB-MP416523FDV
CSB-EP416523FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Recombination protein RecR (recR) CSB-YP416524FDV
CSB-EP416524FDV
CSB-BP416524FDV
CSB-MP416524FDV
CSB-EP416524FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP416525FDV
CSB-EP416525FDV
CSB-BP416525FDV
CSB-MP416525FDV
CSB-EP416525FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Peptide chain release factor 3 (prfC), partial CSB-YP416526FDV
CSB-EP416526FDV
CSB-BP416526FDV
CSB-MP416526FDV
CSB-EP416526FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP416527FDV
CSB-EP416527FDV
CSB-BP416527FDV
CSB-MP416527FDV
CSB-EP416527FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP416528FDV
CSB-EP416528FDV
CSB-BP416528FDV
CSB-MP416528FDV
CSB-EP416528FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica D-alanine--D-alanine ligase (ddl) CSB-YP416529FDV
CSB-EP416529FDV
CSB-BP416529FDV
CSB-MP416529FDV
CSB-EP416529FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii UPF0133 protein Rcas_2292 (Rcas_2292) CSB-YP416530RIP
CSB-EP416530RIP
CSB-BP416530RIP
CSB-MP416530RIP
CSB-EP416530RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Enolase (eno) CSB-YP416531RIP
CSB-EP416531RIP
CSB-BP416531RIP
CSB-MP416531RIP
CSB-EP416531RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Pantothenate synthetase (panC) CSB-YP416532RIP
CSB-EP416532RIP
CSB-BP416532RIP
CSB-MP416532RIP
CSB-EP416532RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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