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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Xanthobacter autotrophicus 50S ribosomal protein L16 (rplP) CSB-YP418333XAV
CSB-EP418333XAV
CSB-BP418333XAV
CSB-MP418333XAV
CSB-EP418333XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus UPF0391 membrane protein Xaut_1725 (Xaut_1725), partial CSB-YP418334XAV
CSB-EP418334XAV
CSB-BP418334XAV
CSB-MP418334XAV
CSB-EP418334XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Protein MraZ (mraZ) CSB-YP418335XAV
CSB-EP418335XAV
CSB-BP418335XAV
CSB-MP418335XAV
CSB-EP418335XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Probable glycine dehydrogenase [decarboxylating] subunit 1 (gcvPA) CSB-YP418336XAV
CSB-EP418336XAV
CSB-BP418336XAV
CSB-MP418336XAV
CSB-EP418336XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus D-amino acid dehydrogenase small subunit (dadA) CSB-YP418337XAV
CSB-EP418337XAV
CSB-BP418337XAV
CSB-MP418337XAV
CSB-EP418337XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Elongation factor P (efp) CSB-YP418338XAV
CSB-EP418338XAV
CSB-BP418338XAV
CSB-MP418338XAV
CSB-EP418338XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus 50S ribosomal protein L25 (rplY) CSB-YP418339XAV
CSB-EP418339XAV
CSB-BP418339XAV
CSB-MP418339XAV
CSB-EP418339XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Probable transcriptional regulatory protein Xaut_3062 (Xaut_3062) CSB-YP418340XAV
CSB-EP418340XAV
CSB-BP418340XAV
CSB-MP418340XAV
CSB-EP418340XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus 30S ribosomal protein S6 (rpsF) CSB-YP418341XAV
CSB-EP418341XAV
CSB-BP418341XAV
CSB-MP418341XAV
CSB-EP418341XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Aspartate--tRNA ligase (aspS), partial CSB-YP418342XAV
CSB-EP418342XAV
CSB-BP418342XAV
CSB-MP418342XAV
CSB-EP418342XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP418343XAV
CSB-EP418343XAV
CSB-BP418343XAV
CSB-MP418343XAV
CSB-EP418343XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus UPF0173 metal-dependent hydrolase Xaut_3786 (Xaut_3786) CSB-YP418344XAV
CSB-EP418344XAV
CSB-BP418344XAV
CSB-MP418344XAV
CSB-EP418344XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP418345XAV
CSB-EP418345XAV
CSB-BP418345XAV
CSB-MP418345XAV
CSB-EP418345XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Isopentenyl-diphosphate delta-isomerase (fni) CSB-YP418346XAV
CSB-EP418346XAV
CSB-BP418346XAV
CSB-MP418346XAV
CSB-EP418346XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Transcriptional repressor NrdR (nrdR) CSB-YP418347XAV
CSB-EP418347XAV
CSB-BP418347XAV
CSB-MP418347XAV
CSB-EP418347XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Glutamate--tRNA ligase 1 (gltX1) CSB-YP418348XAV
CSB-EP418348XAV
CSB-BP418348XAV
CSB-MP418348XAV
CSB-EP418348XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Serine--tRNA ligase (serS) CSB-YP418349XAV
CSB-EP418349XAV
CSB-BP418349XAV
CSB-MP418349XAV
CSB-EP418349XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP418350XAV
CSB-EP418350XAV
CSB-BP418350XAV
CSB-MP418350XAV
CSB-EP418350XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Glutamate--tRNA ligase 2 (gltX2) CSB-YP418351XAV
CSB-EP418351XAV
CSB-BP418351XAV
CSB-MP418351XAV
CSB-EP418351XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus 50S ribosomal protein L30 (rpmD) CSB-YP418352XAV
CSB-EP418352XAV
CSB-BP418352XAV
CSB-MP418352XAV
CSB-EP418352XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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