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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xanthobacter autotrophicus Chaperone protein DnaJ (dnaJ) CSB-YP419033XAV
CSB-EP419033XAV
CSB-BP419033XAV
CSB-MP419033XAV
CSB-EP419033XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP419034XAV
CSB-EP419034XAV
CSB-BP419034XAV
CSB-MP419034XAV
CSB-EP419034XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus FMN-dependent NADH-azoreductase (azoR) CSB-YP419035XAV
CSB-EP419035XAV
CSB-BP419035XAV
CSB-MP419035XAV
CSB-EP419035XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus UPF0262 protein Xaut_1232 (Xaut_1232) CSB-YP419036XAV
CSB-EP419036XAV
CSB-BP419036XAV
CSB-MP419036XAV
CSB-EP419036XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP419037XAV
CSB-EP419037XAV
CSB-BP419037XAV
CSB-MP419037XAV
CSB-EP419037XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus 30S ribosomal protein S10 (rpsJ) CSB-YP419038XAV
CSB-EP419038XAV
CSB-BP419038XAV
CSB-MP419038XAV
CSB-EP419038XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus 50S ribosomal protein L14 (rplN) CSB-YP419039XAV
CSB-EP419039XAV
CSB-BP419039XAV
CSB-MP419039XAV
CSB-EP419039XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Recombination protein RecR (recR) CSB-YP419040XAV
CSB-EP419040XAV
CSB-BP419040XAV
CSB-MP419040XAV
CSB-EP419040XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus 50S ribosomal protein L36 (rpmJ) CSB-YP419041XAV
CSB-EP419041XAV
CSB-BP419041XAV
CSB-MP419041XAV
CSB-EP419041XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Probable hydrogenase nickel incorporation protein HypA (hypA) CSB-YP419042XAV
CSB-EP419042XAV
CSB-BP419042XAV
CSB-MP419042XAV
CSB-EP419042XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP419043XAV
CSB-EP419043XAV
CSB-BP419043XAV
CSB-MP419043XAV
CSB-EP419043XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Phosphoglucosamine mutase (glmM) CSB-YP419044XAV
CSB-EP419044XAV
CSB-BP419044XAV
CSB-MP419044XAV
CSB-EP419044XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus 4-hydroxythreonine-4-phosphate dehydrogenase (pdxA) CSB-YP419045XAV
CSB-EP419045XAV
CSB-BP419045XAV
CSB-MP419045XAV
CSB-EP419045XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Phosphoglycerate kinase (pgk) CSB-YP419046XAV
CSB-EP419046XAV
CSB-BP419046XAV
CSB-MP419046XAV
CSB-EP419046XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP419047XAV
CSB-EP419047XAV
CSB-BP419047XAV
CSB-MP419047XAV
CSB-EP419047XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Fructose-1,6-bisphosphatase class 1 2 (fbp2) CSB-YP419048XAV
CSB-EP419048XAV
CSB-BP419048XAV
CSB-MP419048XAV
CSB-EP419048XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus 50S ribosomal protein L33 (rpmG) CSB-YP419049XAV
CSB-EP419049XAV
CSB-BP419049XAV
CSB-MP419049XAV
CSB-EP419049XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP419050XAV
CSB-EP419050XAV
CSB-BP419050XAV
CSB-MP419050XAV
CSB-EP419050XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Urease accessory protein UreF (ureF) CSB-YP419051XAV
CSB-EP419051XAV
CSB-BP419051XAV
CSB-MP419051XAV
CSB-EP419051XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO CSB-YP419052XAV
CSB-EP419052XAV
CSB-BP419052XAV
CSB-MP419052XAV
CSB-EP419052XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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