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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus amyloliquefaciens Indole-3-glycerol phosphate synthase (trpC) CSB-YP419413BQD
CSB-EP419413BQD
CSB-BP419413BQD
CSB-MP419413BQD
CSB-EP419413BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens GTPase Der (der) CSB-YP419414BQD
CSB-EP419414BQD
CSB-BP419414BQD
CSB-MP419414BQD
CSB-EP419414BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens NADPH dehydrogenase (namA) CSB-YP419415BQD
CSB-EP419415BQD
CSB-BP419415BQD
CSB-MP419415BQD
CSB-EP419415BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Elongation factor P (efp) CSB-YP419416BQD
CSB-EP419416BQD
CSB-BP419416BQD
CSB-MP419416BQD
CSB-EP419416BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Probable endonuclease 4 (nfo) CSB-YP419417BQD
CSB-EP419417BQD
CSB-BP419417BQD
CSB-MP419417BQD
CSB-EP419417BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Chaperone protein DnaK (dnaK), partial CSB-YP419418BQD
CSB-EP419418BQD
CSB-BP419418BQD
CSB-MP419418BQD
CSB-EP419418BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Protein psiE homolog (psiE), partial CSB-YP419419BQD1
CSB-EP419419BQD1
CSB-BP419419BQD1
CSB-MP419419BQD1
CSB-EP419419BQD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Alanine--tRNA ligase (alaS), partial CSB-YP419420BQD
CSB-EP419420BQD
CSB-BP419420BQD
CSB-MP419420BQD
CSB-EP419420BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP419421BQD
CSB-EP419421BQD
CSB-BP419421BQD
CSB-MP419421BQD
CSB-EP419421BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens UPF0758 protein RBAM_025090 (RBAM_025090) CSB-YP419422BQD
CSB-EP419422BQD
CSB-BP419422BQD
CSB-MP419422BQD
CSB-EP419422BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP419423BQD
CSB-EP419423BQD
CSB-BP419423BQD
CSB-MP419423BQD
CSB-EP419423BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Ribonuclease HIII (rnhC) CSB-YP419424BQD
CSB-EP419424BQD
CSB-BP419424BQD
CSB-MP419424BQD
CSB-EP419424BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Acetate kinase (ackA) CSB-YP419425BQD
CSB-EP419425BQD
CSB-BP419425BQD
CSB-MP419425BQD
CSB-EP419425BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens tRNA (guanine-N (7)-)-methyltransferase CSB-YP419426BQD
CSB-EP419426BQD
CSB-BP419426BQD
CSB-MP419426BQD
CSB-EP419426BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP419427BQD
CSB-EP419427BQD
CSB-BP419427BQD
CSB-MP419427BQD
CSB-EP419427BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Homoserine kinase (thrB) CSB-YP419428BQD
CSB-EP419428BQD
CSB-BP419428BQD
CSB-MP419428BQD
CSB-EP419428BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Enolase (eno) CSB-YP419429BQD
CSB-EP419429BQD
CSB-BP419429BQD
CSB-MP419429BQD
CSB-EP419429BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens UPF0042 nucleotide-binding protein RBAM_031990 (RBAM_031990) CSB-YP419430BQD
CSB-EP419430BQD
CSB-BP419430BQD
CSB-MP419430BQD
CSB-EP419430BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP419431BQD1
CSB-EP419431BQD1
CSB-BP419431BQD1
CSB-MP419431BQD1
CSB-EP419431BQD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens D-ribose pyranase (rbsD) CSB-YP419432BQD
CSB-EP419432BQD
CSB-BP419432BQD
CSB-MP419432BQD
CSB-EP419432BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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