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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Thermotoga lettingae Protein grpE (grpE) CSB-YP421315TKE
CSB-EP421315TKE
CSB-BP421315TKE
CSB-MP421315TKE
CSB-EP421315TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae Tryptophan synthase beta chain (trpB) CSB-YP421316TKE
CSB-EP421316TKE
CSB-BP421316TKE
CSB-MP421316TKE
CSB-EP421316TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase (dapH) CSB-YP421317TKE
CSB-EP421317TKE
CSB-BP421317TKE
CSB-MP421317TKE
CSB-EP421317TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae Pseudouridine-5'-phosphate glycosidase (psuG) CSB-YP421318TKE
CSB-EP421318TKE
CSB-BP421318TKE
CSB-MP421318TKE
CSB-EP421318TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae Threonine--tRNA ligase (thrS), partial CSB-YP421319TKE
CSB-EP421319TKE
CSB-BP421319TKE
CSB-MP421319TKE
CSB-EP421319TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus DNA replication and repair protein recF (recF) CSB-YP421320BOL
CSB-EP421320BOL
CSB-BP421320BOL
CSB-MP421320BOL
CSB-EP421320BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Putative septation protein spoVG (spoVG) CSB-YP421321BOL
CSB-EP421321BOL
CSB-BP421321BOL
CSB-MP421321BOL
CSB-EP421321BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus 33 kDa chaperonin (hslO) CSB-YP421322BOL
CSB-EP421322BOL
CSB-BP421322BOL
CSB-MP421322BOL
CSB-EP421322BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus 50S ribosomal protein L1 (rplA) CSB-YP421323BOL
CSB-EP421323BOL
CSB-BP421323BOL
CSB-MP421323BOL
CSB-EP421323BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus 30S ribosomal protein S10 (rpsJ) CSB-YP421324BOL
CSB-EP421324BOL
CSB-BP421324BOL
CSB-MP421324BOL
CSB-EP421324BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus 50S ribosomal protein L22 (rplV) CSB-YP421325BOL
CSB-EP421325BOL
CSB-BP421325BOL
CSB-MP421325BOL
CSB-EP421325BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus 50S ribosomal protein L5 (rplE) CSB-YP421326BOL
CSB-EP421326BOL
CSB-BP421326BOL
CSB-MP421326BOL
CSB-EP421326BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus 50S ribosomal protein L36 (rpmJ) CSB-YP421327BOL
CSB-EP421327BOL
CSB-BP421327BOL
CSB-MP421327BOL
CSB-EP421327BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus L-lactate dehydrogenase (ldh) CSB-YP421328BOL
CSB-EP421328BOL
CSB-BP421328BOL
CSB-MP421328BOL
CSB-EP421328BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Mannitol-1-phosphate 5-dehydrogenase (mtlD) CSB-YP421329BOL
CSB-EP421329BOL
CSB-BP421329BOL
CSB-MP421329BOL
CSB-EP421329BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Serine-protein kinase rsbW (rsbW) CSB-YP421330BOL
CSB-EP421330BOL
CSB-BP421330BOL
CSB-MP421330BOL
CSB-EP421330BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Redox-sensing transcriptional repressor rex (rex) CSB-YP421331BOL
CSB-EP421331BOL
CSB-BP421331BOL
CSB-MP421331BOL
CSB-EP421331BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus UPF0316 protein BPUM_0594 (BPUM_0594), partial CSB-YP421332BOL1
CSB-EP421332BOL1
CSB-BP421332BOL1
CSB-MP421332BOL1
CSB-EP421332BOL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP421333BOL
CSB-EP421333BOL
CSB-BP421333BOL
CSB-MP421333BOL
CSB-EP421333BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Glutamate-1-semialdehyde 2,1-aminomutase 1 (hemL1) CSB-YP421334BOL
CSB-EP421334BOL
CSB-BP421334BOL
CSB-MP421334BOL
CSB-EP421334BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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