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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Bacillus amyloliquefaciens Dihydroorotate dehydrogenase B (NAD (+)), electron transfer subunit CSB-YP422896BQD
CSB-EP422896BQD
CSB-BP422896BQD
CSB-MP422896BQD
CSB-EP422896BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP422897BQD
CSB-EP422897BQD
CSB-BP422897BQD
CSB-MP422897BQD
CSB-EP422897BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens 30S ribosomal protein S16 (rpsP) CSB-YP422898BQD
CSB-EP422898BQD
CSB-BP422898BQD
CSB-MP422898BQD
CSB-EP422898BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens ATP-dependent protease ATPase subunit ClpY (clpY) CSB-YP422899BQD
CSB-EP422899BQD
CSB-BP422899BQD
CSB-MP422899BQD
CSB-EP422899BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Proline--tRNA ligase (proS), partial CSB-YP422900BQD
CSB-EP422900BQD
CSB-BP422900BQD
CSB-MP422900BQD
CSB-EP422900BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Dihydrodipicolinate synthase (dapA) CSB-YP422901BQD
CSB-EP422901BQD
CSB-BP422901BQD
CSB-MP422901BQD
CSB-EP422901BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens DNA mismatch repair protein mutL (mutL), partial CSB-YP422902BQD
CSB-EP422902BQD
CSB-BP422902BQD
CSB-MP422902BQD
CSB-EP422902BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Xylose isomerase (xylA) CSB-YP422903BQD
CSB-EP422903BQD
CSB-BP422903BQD
CSB-MP422903BQD
CSB-EP422903BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP422904BQD1
CSB-EP422904BQD1
CSB-BP422904BQD1
CSB-MP422904BQD1
CSB-EP422904BQD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Aspartate 1-decarboxylase (panD) CSB-YP422905BQD
CSB-EP422905BQD
CSB-BP422905BQD
CSB-MP422905BQD
CSB-EP422905BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP422906BQD
CSB-EP422906BQD
CSB-BP422906BQD
CSB-MP422906BQD
CSB-EP422906BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Chorismate synthase (aroC) CSB-YP422907BQD
CSB-EP422907BQD
CSB-BP422907BQD
CSB-MP422907BQD
CSB-EP422907BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Segregation and condensation protein A (scpA) CSB-YP422908BQD
CSB-EP422908BQD
CSB-BP422908BQD
CSB-MP422908BQD
CSB-EP422908BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens DNA polymerase IV (dinB) CSB-YP422909BQD
CSB-EP422909BQD
CSB-BP422909BQD
CSB-MP422909BQD
CSB-EP422909BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Transcriptional regulator MntR (mntR) CSB-YP422910BQD
CSB-EP422910BQD
CSB-BP422910BQD
CSB-MP422910BQD
CSB-EP422910BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens UPF0178 protein RBAM_023530 (RBAM_023530) CSB-YP422911BQD
CSB-EP422911BQD
CSB-BP422911BQD
CSB-MP422911BQD
CSB-EP422911BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Chaperone protein DnaJ (dnaJ) CSB-YP422912BQD
CSB-EP422912BQD
CSB-BP422912BQD
CSB-MP422912BQD
CSB-EP422912BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) CSB-YP422913BQD
CSB-EP422913BQD
CSB-BP422913BQD
CSB-MP422913BQD
CSB-EP422913BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP422914BQD
CSB-EP422914BQD
CSB-BP422914BQD
CSB-MP422914BQD
CSB-EP422914BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP422915BQD
CSB-EP422915BQD
CSB-BP422915BQD
CSB-MP422915BQD
CSB-EP422915BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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