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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Serratia proteamaculans Phosphopentomutase (deoB) CSB-YP423596STJ
CSB-EP423596STJ
CSB-BP423596STJ
CSB-MP423596STJ
CSB-EP423596STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Isoleucine--tRNA ligase (ileS), partial CSB-YP423597STJ
CSB-EP423597STJ
CSB-BP423597STJ
CSB-MP423597STJ
CSB-EP423597STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP423598STJ
CSB-EP423598STJ
CSB-BP423598STJ
CSB-MP423598STJ
CSB-EP423598STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Protein MraZ (mraZ) CSB-YP423599STJ
CSB-EP423599STJ
CSB-BP423599STJ
CSB-MP423599STJ
CSB-EP423599STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans GMP reductase (guaC) CSB-YP423600STJ
CSB-EP423600STJ
CSB-BP423600STJ
CSB-MP423600STJ
CSB-EP423600STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Enolase (eno) CSB-YP423601STJ
CSB-EP423601STJ
CSB-BP423601STJ
CSB-MP423601STJ
CSB-EP423601STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans tRNA pseudouridine synthase D (truD) CSB-YP423602STJ
CSB-EP423602STJ
CSB-BP423602STJ
CSB-MP423602STJ
CSB-EP423602STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Alanine--tRNA ligase (alaS), partial CSB-YP423603STJ
CSB-EP423603STJ
CSB-BP423603STJ
CSB-MP423603STJ
CSB-EP423603STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Na (+)-translocating NADH-quinone reductase subunit E, partial CSB-YP423604STJ1
CSB-EP423604STJ1
CSB-BP423604STJ1
CSB-MP423604STJ1
CSB-EP423604STJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Sigma factor-binding protein crl (crl) CSB-YP423605STJ
CSB-EP423605STJ
CSB-BP423605STJ
CSB-MP423605STJ
CSB-EP423605STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans UPF0345 protein Spro_1026 (Spro_1026) CSB-YP423606STJ
CSB-EP423606STJ
CSB-BP423606STJ
CSB-MP423606STJ
CSB-EP423606STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP423607STJ
CSB-EP423607STJ
CSB-BP423607STJ
CSB-MP423607STJ
CSB-EP423607STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP423608STJ
CSB-EP423608STJ
CSB-BP423608STJ
CSB-MP423608STJ
CSB-EP423608STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Adenylate kinase (adk) CSB-YP423609STJ
CSB-EP423609STJ
CSB-BP423609STJ
CSB-MP423609STJ
CSB-EP423609STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Octanoyltransferase (lipB) CSB-YP423610STJ
CSB-EP423610STJ
CSB-BP423610STJ
CSB-MP423610STJ
CSB-EP423610STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP423611STJ
CSB-EP423611STJ
CSB-BP423611STJ
CSB-MP423611STJ
CSB-EP423611STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Protein TolB (tolB) CSB-YP423612STJ
CSB-EP423612STJ
CSB-BP423612STJ
CSB-MP423612STJ
CSB-EP423612STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Biotin synthase (bioB) CSB-YP423613STJ
CSB-EP423613STJ
CSB-BP423613STJ
CSB-MP423613STJ
CSB-EP423613STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Betaine aldehyde dehydrogenase (betB) CSB-YP423614STJ
CSB-EP423614STJ
CSB-BP423614STJ
CSB-MP423614STJ
CSB-EP423614STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP423615STJ
CSB-EP423615STJ
CSB-BP423615STJ
CSB-MP423615STJ
CSB-EP423615STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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