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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia multivorans 30S ribosomal protein S4 (rpsD) CSB-YP430801BPS
CSB-EP430801BPS
CSB-BP430801BPS
CSB-MP430801BPS
CSB-EP430801BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 30S ribosomal protein S16 (rpsP) CSB-YP430802BPS
CSB-EP430802BPS
CSB-BP430802BPS
CSB-MP430802BPS
CSB-EP430802BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP430803BPS
CSB-EP430803BPS
CSB-BP430803BPS
CSB-MP430803BPS
CSB-EP430803BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Octanoyltransferase (lipB) CSB-YP430804BPS
CSB-EP430804BPS
CSB-BP430804BPS
CSB-MP430804BPS
CSB-EP430804BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Probable septum site-determining protein MinC (minC) CSB-YP430805BPS
CSB-EP430805BPS
CSB-BP430805BPS
CSB-MP430805BPS
CSB-EP430805BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans UPF0042 nucleotide-binding protein Bmul_0520/BMULJ_02739 (Bmul_0520, BMULJ_02739) CSB-YP430806BPS
CSB-EP430806BPS
CSB-BP430806BPS
CSB-MP430806BPS
CSB-EP430806BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Urease accessory protein UreF (ureF) CSB-YP430807BPS
CSB-EP430807BPS
CSB-BP430807BPS
CSB-MP430807BPS
CSB-EP430807BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Glycerol kinase (glpK) CSB-YP430808BPS
CSB-EP430808BPS
CSB-BP430808BPS
CSB-MP430808BPS
CSB-EP430808BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Transcriptional repressor NrdR (nrdR) CSB-YP430809BPS
CSB-EP430809BPS
CSB-BP430809BPS
CSB-MP430809BPS
CSB-EP430809BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP430810BPS
CSB-EP430810BPS
CSB-BP430810BPS
CSB-MP430810BPS
CSB-EP430810BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Chaperone protein DnaK (dnaK), partial CSB-YP430811BPS
CSB-EP430811BPS
CSB-BP430811BPS
CSB-MP430811BPS
CSB-EP430811BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP430812BPS
CSB-EP430812BPS
CSB-BP430812BPS
CSB-MP430812BPS
CSB-EP430812BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP430813BPS
CSB-EP430813BPS
CSB-BP430813BPS
CSB-MP430813BPS
CSB-EP430813BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Bifunctional protein FolD (folD) CSB-YP430814BPS
CSB-EP430814BPS
CSB-BP430814BPS
CSB-MP430814BPS
CSB-EP430814BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Histidine--tRNA ligase (hisS) CSB-YP430815BPS
CSB-EP430815BPS
CSB-BP430815BPS
CSB-MP430815BPS
CSB-EP430815BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP430816BPS
CSB-EP430816BPS
CSB-BP430816BPS
CSB-MP430816BPS
CSB-EP430816BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 50S ribosomal protein L28 (rpmB) CSB-YP430817BPS
CSB-EP430817BPS
CSB-BP430817BPS
CSB-MP430817BPS
CSB-EP430817BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP430818BPS
CSB-EP430818BPS
CSB-BP430818BPS
CSB-MP430818BPS
CSB-EP430818BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Proline--tRNA ligase (proS), partial CSB-YP430819BPS
CSB-EP430819BPS
CSB-BP430819BPS
CSB-MP430819BPS
CSB-EP430819BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Chromosomal replication initiator protein DnaA (dnaA), partial CSB-YP430820BPS
CSB-EP430820BPS
CSB-BP430820BPS
CSB-MP430820BPS
CSB-EP430820BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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