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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus weihenstephanensis Cell cycle protein GpsB (gpsB) CSB-YP433302BON
CSB-EP433302BON
CSB-BP433302BON
CSB-MP433302BON
CSB-EP433302BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Probable cytosol aminopeptidase (pepA) CSB-YP433303BON
CSB-EP433303BON
CSB-BP433303BON
CSB-MP433303BON
CSB-EP433303BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Putative ATP:guanido phosphotransferase BcerKBAB4_0075 (BcerKBAB4_0075) CSB-YP433304BON
CSB-EP433304BON
CSB-BP433304BON
CSB-MP433304BON
CSB-EP433304BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Cysteine--tRNA ligase (cysS) CSB-YP433305BON
CSB-EP433305BON
CSB-BP433305BON
CSB-MP433305BON
CSB-EP433305BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP433306BON
CSB-EP433306BON
CSB-BP433306BON
CSB-MP433306BON
CSB-EP433306BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 50S ribosomal protein L22 (rplV) CSB-YP433307BON
CSB-EP433307BON
CSB-BP433307BON
CSB-MP433307BON
CSB-EP433307BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 30S ribosomal protein S17 (rpsQ) CSB-YP433308BON
CSB-EP433308BON
CSB-BP433308BON
CSB-MP433308BON
CSB-EP433308BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 30S ribosomal protein S14 type Z (rpsZ) CSB-YP433309BON
CSB-EP433309BON
CSB-BP433309BON
CSB-MP433309BON
CSB-EP433309BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 50S ribosomal protein L17 (rplQ) CSB-YP433310BON
CSB-EP433310BON
CSB-BP433310BON
CSB-MP433310BON
CSB-EP433310BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Pyrophosphatase ppaX (ppaX) CSB-YP433311BON
CSB-EP433311BON
CSB-BP433311BON
CSB-MP433311BON
CSB-EP433311BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Redox-sensing transcriptional repressor rex (rex) CSB-YP433312BON
CSB-EP433312BON
CSB-BP433312BON
CSB-MP433312BON
CSB-EP433312BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Phosphomethylpyrimidine synthase (thiC), partial CSB-YP433313BON
CSB-EP433313BON
CSB-BP433313BON
CSB-MP433313BON
CSB-EP433313BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 1-pyrroline-5-carboxylate dehydrogenase (rocA), partial CSB-YP433314BON
CSB-EP433314BON
CSB-BP433314BON
CSB-MP433314BON
CSB-EP433314BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Thiamine-phosphate synthase (thiE) CSB-YP433315BON
CSB-EP433315BON
CSB-BP433315BON
CSB-MP433315BON
CSB-EP433315BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP433316BON
CSB-EP433316BON
CSB-BP433316BON
CSB-MP433316BON
CSB-EP433316BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UPF0741 protein BcerKBAB4_5177 (BcerKBAB4_5177) CSB-YP433317BON
CSB-EP433317BON
CSB-BP433317BON
CSB-MP433317BON
CSB-EP433317BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Ribonuclease HII (rnhB) CSB-YP433318BON
CSB-EP433318BON
CSB-BP433318BON
CSB-MP433318BON
CSB-EP433318BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 50S ribosomal protein L28 (rpmB) CSB-YP433319BON
CSB-EP433319BON
CSB-BP433319BON
CSB-MP433319BON
CSB-EP433319BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UPF0296 protein BcerKBAB4_3695 (BcerKBAB4_3695) CSB-YP433320BON
CSB-EP433320BON
CSB-BP433320BON
CSB-MP433320BON
CSB-EP433320BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Lipoprotein signal peptidase (lspA), partial CSB-YP433321BON1
CSB-EP433321BON1
CSB-BP433321BON1
CSB-MP433321BON1
CSB-EP433321BON1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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