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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia multivorans UPF0271 protein Bmul_3034/BMULJ_00198 (Bmul_3034, BMULJ_00198) CSB-YP435163BPS
CSB-EP435163BPS
CSB-BP435163BPS
CSB-MP435163BPS
CSB-EP435163BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP435164BPS
CSB-EP435164BPS
CSB-BP435164BPS
CSB-MP435164BPS
CSB-EP435164BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Elongation factor P (efp) CSB-YP435165BPS
CSB-EP435165BPS
CSB-BP435165BPS
CSB-MP435165BPS
CSB-EP435165BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 50S ribosomal protein L1 (rplA) CSB-YP435166BPS
CSB-EP435166BPS
CSB-BP435166BPS
CSB-MP435166BPS
CSB-EP435166BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 50S ribosomal protein L23 (rplW) CSB-YP435167BPS
CSB-EP435167BPS
CSB-BP435167BPS
CSB-MP435167BPS
CSB-EP435167BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 30S ribosomal protein S3 (rpsC) CSB-YP435168BPS
CSB-EP435168BPS
CSB-BP435168BPS
CSB-MP435168BPS
CSB-EP435168BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 30S ribosomal protein S5 (rpsE) CSB-YP435169BPS
CSB-EP435169BPS
CSB-BP435169BPS
CSB-MP435169BPS
CSB-EP435169BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Probable GTP-binding protein EngB (engB) CSB-YP435170BPS
CSB-EP435170BPS
CSB-BP435170BPS
CSB-MP435170BPS
CSB-EP435170BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans ADP-L-glycero-D-manno-heptose-6-epimerase (hldD) CSB-YP435171BPS
CSB-EP435171BPS
CSB-BP435171BPS
CSB-MP435171BPS
CSB-EP435171BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP435172BPS
CSB-EP435172BPS
CSB-BP435172BPS
CSB-MP435172BPS
CSB-EP435172BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP435173BPS
CSB-EP435173BPS
CSB-BP435173BPS
CSB-MP435173BPS
CSB-EP435173BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Phosphopantetheine adenylyltransferase (coaD) CSB-YP435174BPS
CSB-EP435174BPS
CSB-BP435174BPS
CSB-MP435174BPS
CSB-EP435174BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP435175BPS
CSB-EP435175BPS
CSB-BP435175BPS
CSB-MP435175BPS
CSB-EP435175BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP435176BPS
CSB-EP435176BPS
CSB-BP435176BPS
CSB-MP435176BPS
CSB-EP435176BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Protein RecA (recA) CSB-YP435177BPS
CSB-EP435177BPS
CSB-BP435177BPS
CSB-MP435177BPS
CSB-EP435177BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP435178BPS1
CSB-EP435178BPS1
CSB-BP435178BPS1
CSB-MP435178BPS1
CSB-EP435178BPS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP435179BPS
CSB-EP435179BPS
CSB-BP435179BPS
CSB-MP435179BPS
CSB-EP435179BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP435180BPS
CSB-EP435180BPS
CSB-BP435180BPS
CSB-MP435180BPS
CSB-EP435180BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Lipoprotein signal peptidase (lspA), partial CSB-YP435181BPS1
CSB-EP435181BPS1
CSB-BP435181BPS1
CSB-MP435181BPS1
CSB-EP435181BPS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP435182BPS
CSB-EP435182BPS
CSB-BP435182BPS
CSB-MP435182BPS
CSB-EP435182BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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