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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharomyces cerevisiae Regulator of rDNA transcription protein 5 (RRT5) CSB-YP455378SVP
CSB-EP455378SVP
CSB-BP455378SVP
CSB-MP455378SVP
CSB-EP455378SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae WD repeat-containing protein 55 homolog (GF18399) CSB-YP455379DKY
CSB-EP455379DKY
CSB-BP455379DKY
CSB-MP455379DKY
CSB-EP455379DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Probable ATP-dependent RNA helicase spindle-E (spn-E), partial CSB-YP455380DKY
CSB-EP455380DKY
CSB-BP455380DKY
CSB-MP455380DKY
CSB-EP455380DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Protein maelstrom 1 (mael1) CSB-YP455381DKY
CSB-EP455381DKY
CSB-BP455381DKY
CSB-MP455381DKY
CSB-EP455381DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Lissencephaly-1 homolog (Lis-1) CSB-YP455382DKY
CSB-EP455382DKY
CSB-BP455382DKY
CSB-MP455382DKY
CSB-EP455382DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Latrophilin Cirl (Cirl), partial CSB-YP455383DKY
CSB-EP455383DKY
CSB-BP455383DKY
CSB-MP455383DKY
CSB-EP455383DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Interference hedgehog (iHog), partial CSB-YP455384DKY
CSB-EP455384DKY
CSB-BP455384DKY
CSB-MP455384DKY
CSB-EP455384DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Probable cGMP 3',5'-cyclic phosphodiesterase subunit delta (PrBP) CSB-YP455385DKY
CSB-EP455385DKY
CSB-BP455385DKY
CSB-MP455385DKY
CSB-EP455385DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Neuropathy target esterase sws (sws), partial CSB-YP455386DKY
CSB-EP455386DKY
CSB-BP455386DKY
CSB-MP455386DKY
CSB-EP455386DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Eukaryotic translation initiation factor 3 subunit G-2 (eIF3-S4-2) CSB-YP455387DKY
CSB-EP455387DKY
CSB-BP455387DKY
CSB-MP455387DKY
CSB-EP455387DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae tRNA (guanine-N (7)-)-methyltransferase CSB-YP455388DKY
CSB-EP455388DKY
CSB-BP455388DKY
CSB-MP455388DKY
CSB-EP455388DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Interference hedgehog (iHog), partial CSB-YP455389DLK
CSB-EP455389DLK
CSB-BP455389DLK
CSB-MP455389DLK
CSB-EP455389DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Probable cGMP 3',5'-cyclic phosphodiesterase subunit delta (PrBP) CSB-YP455390DLK
CSB-EP455390DLK
CSB-BP455390DLK
CSB-MP455390DLK
CSB-EP455390DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Ell-associated factor Eaf (Eaf), partial CSB-YP455391DLK
CSB-EP455391DLK
CSB-BP455391DLK
CSB-MP455391DLK
CSB-EP455391DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Queuine tRNA-ribosyltransferase subunit QTRTD1 homolog (GG14034) CSB-YP455392DLK
CSB-EP455392DLK
CSB-BP455392DLK
CSB-MP455392DLK
CSB-EP455392DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Protein alan shepard (shep), partial CSB-YP455393DLK
CSB-EP455393DLK
CSB-BP455393DLK
CSB-MP455393DLK
CSB-EP455393DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Protein crossbronx-like (GG20882) CSB-YP455394DLK
CSB-EP455394DLK
CSB-BP455394DLK
CSB-MP455394DLK
CSB-EP455394DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Dynein assembly factor 1, axonemal homolog (dtr), partial CSB-YP455395DLK
CSB-EP455395DLK
CSB-BP455395DLK
CSB-MP455395DLK
CSB-EP455395DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Cytosolic Fe-S cluster assembly factor NUBP1 homolog (GG22765) CSB-YP455396DLK
CSB-EP455396DLK
CSB-BP455396DLK
CSB-MP455396DLK
CSB-EP455396DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Lissencephaly-1 homolog (Lis-1) CSB-YP455397DLK
CSB-EP455397DLK
CSB-BP455397DLK
CSB-MP455397DLK
CSB-EP455397DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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