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  4. Bovine Beta-Endorphin,β-EP ELISA Kit

Bovine Beta-Endorphin,β-EP ELISA Kit

  • 中文名稱：
    牛β內(nèi)啡肽(β-EP)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13051B
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    牛β內(nèi)啡肽(β-EP)酶聯(lián)免疫試劑盒（CSB-E13051B）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的β-EP含量。β - EP即β - 內(nèi)啡肽，是人體自身產(chǎn)生的具有強(qiáng)大鎮(zhèn)痛作用的內(nèi)源性活性物質(zhì)。其研究機(jī)制主要圍繞它與體內(nèi)阿片受體結(jié)合，調(diào)節(jié)痛覺(jué)傳導(dǎo)、情緒等生理過(guò)程，在鎮(zhèn)痛、精神疾病等領(lǐng)域研究較多，對(duì)理解人體生理和開(kāi)發(fā)相關(guān)療法有重要意義。試劑盒檢測(cè)范圍為46.88 pg/mL-3000 pg/mL，可為神經(jīng)生物學(xué)、疼痛機(jī)制研究或應(yīng)激反應(yīng)動(dòng)物模型提供數(shù)據(jù)支持，例如探究藥物干預(yù)、環(huán)境刺激對(duì)β - EP表達(dá)水平的影響；適用于牛源樣本的體外定量分析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    beta-Endorphin, beta-EP
  • 縮寫(xiě)：
    β-EP
  • 種屬：
    Bos taurus (Bovine)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    46.88 pg/mL-3000 pg/mL
  • 靈敏度：
    11.72 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %95
    Range %90-99
    1:2Average %84
    Range %80-88
    1:4Average %92
    Range %88-97
    1:8Average %90
    Range %85-94
  • 回收率：
    The recovery of bovine β-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9995-104
    EDTA plasma (n=4)9288-96
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    30001.701 1.612 1.657 1.559
    15001.376 1.458 1.417 1.319
    7501.113 1.104 1.109 1.011
    3750.832 0.809 0.821 0.723
    187.50.524 0.560 0.542 0.444
    93.750.367 0.359 0.363 0.265
    46.880.264 0.251 0.258 0.160
    00.099 0.097 0.098 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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