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Human Beta-Endorphin,β-EP ELISA Kit

  • 中文名稱:
    人β內(nèi)啡肽(β-EP)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E06821h
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人β內(nèi)啡肽(β-EP)酶聯(lián)免疫試劑盒(CSB-E06821h)為雙抗夾心法ELISA試劑盒,定量檢測血清、尿液、腦脊液(CSF)、唾液、組織勻漿樣本中的β-EP含量。β-EP(β-內(nèi)啡肽)是體內(nèi)天然阿片肽,在痛覺調(diào)節(jié)、情緒調(diào)控等方面有重要作用。研究機制上,其能與體內(nèi)阿片受體結(jié)合,產(chǎn)生鎮(zhèn)痛、愉悅感等效果。對它的研究有助于深入了解疼痛、成癮及情緒相關(guān)疾病發(fā)病機制與治療。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL,適用于神經(jīng)生物學(xué)、疼痛機制及應(yīng)激反應(yīng)等基礎(chǔ)研究領(lǐng)域;其水平變化與神經(jīng)退行性疾病、慢性疼痛綜合征及精神類疾病的研究密切相關(guān);支持科研人員對中樞神經(jīng)系統(tǒng)、內(nèi)分泌系統(tǒng)及相關(guān)病理模型的深入分析;樣本兼容性涵蓋體液與組織裂解液,特別適用于跨樣本類型的對比研究,例如腦脊液與血清中β-EP的動態(tài)關(guān)聯(lián)分析,或特定腦區(qū)組織勻漿的局部濃度檢測;為探索β-EP在神經(jīng)信號傳導(dǎo)、應(yīng)激適應(yīng)機制及藥物干預(yù)效應(yīng)等方向提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    beta-Endorphin, beta-EP
  • 縮寫:
    β-EP
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, urine, cerebrospinal fluid (CSF), saliva, tissue homogenates
  • 檢測范圍:
    62.5 pg/mL-4000 pg/mL
  • 靈敏度:
    15.6 pg/mL
  • 反應(yīng)時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Cancer
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %86
    Range %80-92
    1:2Average %99
    Range %91-110
    1:4Average %97
    Range %92-105
    1:8Average %93
    Range %86-98
  • 回收率:
    The recovery of human β-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-99
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    40002.537 2.568 2.553 2.443
    20002.010 1.999 2.005 1.895
    10001.600 1.532 1.566 1.456
    5001.032 1.122 1.077 0.967
    2500.567 0.600 0.584 0.474
    1250.391 0.410 0.401 0.291
    62.50.242 0.254 0.248 0.138
    00.109 0.111 0.110  
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評價

相關(guān)問答

 常見問題解答
Q:

我想咨詢一下人類β-內(nèi)啡肽ELISA試劑盒(CSB-E06821h)的問題。在規(guī)格說明書和使用手冊中,我不理解“靈敏度15.6 pg/mL”和“檢測范圍為62.5 pg/mL至4,000 pg/mL”的差異。在這個ELISA試劑盒中,15.6 pg/mL是最低可檢測水平嗎?如果是,為什么最低可檢測水平(15.6 pg/mL)低于范圍的最低水平(62.5 pg/mL)?

A:
L靈敏度15.6pg/m是該試劑盒的最低檢測水平,是通過重復(fù)測定20次零標準品OD值及其三倍標準差得出的。低于此參數(shù)(15.6pg/mL)則無法檢測。在15.6-62.5pg/mL區(qū)間范圍,樣品的測量值具有很大的變異性。62.5pg/mL 表示標準品S1的濃度點,而62.5-4000pg/mL是該試劑盒的檢測范圍。