Bovine Thyroid Stimulating Hormone(TSH) ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

牛促甲狀腺素(TSH)酶聯(lián)免疫試劑盒（CSB-E17548B）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TSH含量。TSH即促甲狀腺激素，是腺垂體分泌的促進(jìn)甲狀腺生長和機(jī)能的激素。其可調(diào)控甲狀腺細(xì)胞的增殖與甲狀腺血液供應(yīng)。研究多圍繞其與甲狀腺疾病如甲亢、甲減的關(guān)系展開，明確其作用機(jī)制有助于疾病的診斷、治療及病情監(jiān)測。試劑盒檢測范圍為0.9 uIU/mL-60 uIU/mL，適用于牛甲狀腺相關(guān)疾病機(jī)制研究、藥物干預(yù)效果評估或畜牧養(yǎng)殖中生理狀態(tài)監(jiān)測等科研場景本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

TSHB ELISA Kit; Thyrotropin subunit beta ELISA Kit; Thyroid-stimulating hormone subunit beta ELISA Kit; TSH-B ELISA Kit; TSH-beta ELISA Kit; Thyrotropin beta chain ELISA Kit
縮寫：

TSH
Uniprot No.：

P01223
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.9 uIU/mL-60 uIU/mL
靈敏度：

0.225 μIU/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine TSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-101
1:2	Average %	88
	Range %	84-92
1:4	Average %	90
	Range %	85-98
1:8	Average %	92
	Range %	88-96

回收率：

The recovery of bovine TSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	95-104
EDTA plasma (n=4)	84	80-88

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Indispensable for the control of thyroid structure and metabolism.
基因功能參考文獻(xiàn)：
1. Nitric oxide decreases TSH-induced FoxE1 expression, thus repressing the transcripcional activation of TPO gene. PMID: 26610751
2. identification for the first time that ADAM10 is a TSHR cleavage enzyme and demonstration that TSH regulates its activation PMID: 18074395
亞細(xì)胞定位：

Secreted.
蛋白家族：

Glycoprotein hormones subunit beta family
數(shù)據(jù)庫鏈接：

KEGG: bta:281552

STRING: 9913.ENSBTAP00000008264

UniGene: Bt.475