Canine Dihydrotestosterone,DHT ELISA Kit

產品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

犬雙氫睪酮(DHT)酶聯(lián)免疫試劑盒（CSB-E07881c）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的DHT含量。DHT即雙氫睪酮，是睪酮在5α-還原酶作用下轉化而來。它與雄激素性脫發(fā)、前列腺增生等疾病密切相關。研究機制方面，主要聚焦其和靶細胞內雄激素受體結合，影響基因表達，促進細胞生長分化，目前也是相關疾病藥物研發(fā)重要靶點。試劑盒檢測范圍為40 pg/mL-8000 pg/mL，該產品適用于動物實驗中對激素干預效果評估、生殖健康研究或代謝性疾病模型的 DHT 動態(tài)分析，例如前列腺增生、脫發(fā)機制或內分泌紊亂相關研究，為探索犬類生理病理機制提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

DHT
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

40 pg/mL-8000 pg/mL
靈敏度：

20 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine DHT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	93-103
1:2	Average %	95
	Range %	91-99
1:4	Average %	102
	Range %	98-104
1:8	Average %	89
	Range %	82-95

回收率：

The recovery of canine DHT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	99	98-103

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.