Human Dihydrotestosterone,DHT ELISA Kit

產(chǎn)品詳情

文獻引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人雙氫睪酮(DHT)酶聯(lián)免疫試劑盒（CSB-E07878h）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的DHT含量。DHT即雙氫睪酮，是睪酮經(jīng)5α-還原酶作用轉(zhuǎn)化而來。它與雄激素性脫發(fā)、前列腺增生等密切相關。研究機制主要聚焦于其與雄激素受體結合，影響毛囊、前列腺等組織細胞的生長、分化，為相關疾病的藥物研發(fā)提供方向。試劑盒檢測范圍為10 pg/mL-2000 pg/mL，本產(chǎn)品可廣泛應用于激素作用機制研究、內(nèi)分泌系統(tǒng)功能評估、前列腺相關疾病模型構建及藥物干預效果分析等科研領域，為探索DHT在生理病理過程中的調(diào)控網(wǎng)絡提供高效工具，適用于細胞實驗、動物模型及臨床前研究的數(shù)據(jù)獲取。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

DHT
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

10 pg/mL-2000 pg/mL
靈敏度：

5 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human DHT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-97
1:2	Average %	89
	Range %	85-99
1:4	Average %	93
	Range %	86-105
1:8	Average %	94
	Range %	87-103

回收率：

The recovery of human DHT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-99
EDTA plasma (n=4)	97	92-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.