Canine Progesterone,PROG ELISA kit
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中文名稱:犬孕激素/孕酮(PROG)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E07285c
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規(guī)格:96T/48T
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價(jià)格:¥4200/¥3000
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:犬孕激素/孕酮(PROG)酶聯(lián)免疫試劑盒(CSB-E07285c)為競(jìng)爭(zhēng)法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿.樣本中的PROG含量。試劑盒檢測(cè)范圍為0.15 ng/ml-70 ng/ml,靈敏度為0.2 ng/ml。適用于動(dòng)物繁殖生理研究、生殖內(nèi)分泌機(jī)制探索、藥物對(duì)卵巢功能影響評(píng)估等場(chǎng)景,尤其可為犬類人工授精時(shí)機(jī)判斷、妊娠監(jiān)測(cè)及生殖疾病模型構(gòu)建提供可靠數(shù)據(jù)支持本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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縮寫:PROG
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種屬:Canis lupus familiaris (Dog) (Canis familiaris)
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樣本類型:serum, plasma, tissue homogenates.
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檢測(cè)范圍:0.15 ng/ml-70 ng/ml.
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靈敏度:0.2 ng/ml.
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Signal Transduction
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測(cè)定原理:quantitative
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測(cè)定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of canine PROG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
96
Range %
92-104
1:2
Average %
94
Range %
91-99
1:4
Average %
102
Range %
98-105
1:8
Average %
89
Range %
82-94
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回收率:
The recovery of canine PROG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
93
90-97
EDTA plasma (n=4)
101
98-105
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標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml
OD1
OD2
Average
0
2.284
2.275
2.280
0.15
1.429
1.589
1.509
1
0.843
0.874
0.859
5
0.566
0.585
0.576
20
0.362
0.353
0.358
70
0.201
0.202
0.202
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數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- The evaluation of the oxidative stress index of reproductive tissues and serum thiol/disulphide homeostasis during estrous cycle in bitches Acar D B,Ankara ?niversitesi Veteriner Fak眉ltesi Dergisi,2019
- Anti-Müllerian hormone as a diagnostic tool for ovarian remnant syndrome in bitches. Turna Yilmaz Ö. et al,Vet Res Commun,2015
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