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  4. Canine acetylcholine receptor antibody,AChRab ELISA Kit

Canine acetylcholine receptor antibody,AChRab ELISA Kit

  • 中文名稱：
    犬乙酰膽堿受體抗體(AChRab)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E08375c
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4600/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    犬乙酰膽堿受體抗體(AChRab)酶聯(lián)免疫試劑盒（CSB-E08375c）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的AChR Ab含量。AChR Ab即乙酰膽堿受體抗體，它與重癥肌無(wú)力等疾病密切相關(guān)。其背景在于機(jī)體免疫系統(tǒng)異常產(chǎn)生該抗體。研究機(jī)制主要是它會(huì)與神經(jīng)肌肉接頭處的乙酰膽堿受體結(jié)合，干擾神經(jīng)沖動(dòng)傳導(dǎo)，影響肌肉正常收縮，是相關(guān)疾病發(fā)病的重要因素。試劑盒檢測(cè)范圍為3.75 ng/mL-240 ng/mL，適用于基礎(chǔ)科研中探索AChRab在神經(jīng)功能障礙模型中的表達(dá)變化，或用于藥物開(kāi)發(fā)過(guò)程中評(píng)估實(shí)驗(yàn)性治療對(duì)抗體水平的調(diào)控作用，同時(shí)也可作為犬類自身免疫疾病研究中的生物標(biāo)志物檢測(cè)方案，為動(dòng)物模型的建立及病理機(jī)制解析提供數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 縮寫：
    AChR Ab
  • 種屬：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 樣本類型：
    serum, plasma
  • 檢測(cè)范圍：
    3.75 ng/mL-240 ng/mL
  • 靈敏度：
    0.94 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of canine AChRab in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 89-102  
    1:2 Average % 95  
    Range % 84-100  
    1:4 Average % 96  
    Range % 90-103  
    1:8 Average % 94  
    Range % 82-101  
  • 回收率：
    The recovery of canine AChRab spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-101  
    EDTA plasma (n=4) 92 87-99  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    240 2.954 2.918 2.936 2.832  
    120 2.512 2.631 2.572 2.468  
    60 1.852 1.873 1.863 1.759  
    30 1.034 0.996 1.015 0.911  
    15 0.472 0.494 0.483 0.379  
    7.5 0.261 0.276 0.269 0.165  
    3.75 0.155 0.161 0.158 0.054  
    0 0.102 0.106 0.104 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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相關(guān)問(wèn)答

■ 常見(jiàn)問(wèn)題解答
Q:

I want to use this kit to test the possibility of myasthenia gravis.

A:
Thanks for your inquiry. It is ok to test possibility of myasthenia gravis but we did not ever validate it. Pls follow your actual test result.