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  4. Human acetylcholine receptor antibody,AChRab ELISA Kit

Human acetylcholine receptor antibody,AChRab ELISA Kit

  • 中文名稱：
    人乙酰膽堿受體抗體(AChRab)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08371h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人乙酰膽堿受體抗體(AChRab)酶聯(lián)免疫試劑盒（CSB-E08371h）為間接法ELISA試劑盒，定性檢測血清樣本中的AChR Ab含量。AChR Ab即乙酰膽堿受體抗體。它與重癥肌無力等自身免疫性疾病密切相關(guān)，是重要研究靶點(diǎn)。其研究機(jī)制主要聚焦于它與乙酰膽堿受體結(jié)合后，干擾神經(jīng)肌肉接頭處信號傳導(dǎo)，導(dǎo)致肌肉無力等癥狀，為相關(guān)疾病診斷與治療提供依據(jù)。適用于重癥肌無力等疾病的發(fā)病機(jī)制研究、藥物干預(yù)效果評估或?qū)嶒?yàn)動物模型抗體水平檢測等科研場景本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    AChR Ab
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum
  • 檢測范圍：
    Request Information
  • 靈敏度：
    Request Information
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測定原理：
    qualitative
  • 測定方法：
    Indirect
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<15%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<15%

    Three samples of known concentration were tested in twenty assays to assess.

  • 標(biāo)準(zhǔn)曲線：

    Test parameter

    specification

    test result

    Positive control

    >0.8

    0.834

    Negative control

    <0.2

    0.047

    Positive rate

    10，Positive

    100%

    Negative rate

    10，Negative

    100%

  • 本試劑盒所含材料：
    • A 96-well Assay plate --The 96-well plate has been pre-coated with AChR.
    • Negative Control (1 x 1ml) --Eliminate false positive
    • Positive Control (1 x 1ml) --Used to evaluate the validity, stability, and comparability of experimental results.
    • Sample Diluent (1x 20 ml) --Dilute the sample to an appropriate concentration.
    • HRP-conjugated anti-human IgG (1 x 10ml)--Bind to the AChRab, and HRP catalyzes the TMB to elicit a chromogenic reaction.
    • Wash Buffer (25x concentrate) (1 x 20 ml) --Wash away unbound or free substances.
    • Substrate A (1 x 5 ml) --Mix with substrate B and interact with HRP, eliciting a Chromogenic reaction.
    • Substrate B (1 x 5 ml) --Mix with substrate A and interact with HRP, eliciting a Chromogenic reaction.
    • Stop Solution (1 x 5 ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells)--Cover the microplate when incubation.
    • An Instruction manual

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  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 貨期：
    3-5 working days

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相關(guān)問答

■ 常見問題解答
Q:

Under storage section: It has mentioned that opened kit can be stored at 2-8 degrees upto 2 weeks( in case of AcH R) and only upto one month ( in case of MuSk kit)---These are special tests which we can't consume opend kit in 2 weeks or 1 month) minimum it should work 5-6 months( Please confirm how many months we can use opened kit with proper storage and handling of the kit)

A:
Thanks for your inquiry. For the storage condition, pls follow the manual.
Regarding CSB-E08371h, you need to set 6 wells including control,blank wells.Thus the kit can test 90 samples.If you set duplicates,then it can test 45 samples.Based on the existing experiment condition,we suggest you try your best to collect samples as much as possible to avoid any affects to the testing results due to the overdue.
Our elisa kits are only for research use,not for IVD use.There is no industrial standards for kits for research use and we do not have CE certificate.We only have ISO 9001 certificate.
If you need to do seperate assays with one kit during long period,we suggest you order small size,such as 24T or 48T.But you can only order qty 1 for the same item of 24T.
If further questions,pls let me know.