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  4. Canine kidney injury molecule 1(Kim-1) ELISA Kit

Canine kidney injury molecule 1(Kim-1) ELISA Kit

  • 中文名稱：
    犬腎損傷分子1(Kim-1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E13035c
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    犬腎損傷分子1(Kim-1)酶聯(lián)免疫試劑盒（CSB-E13035c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Kim-1含量。Kim-1即腎損傷分子-1，是腎小管上皮細胞在腎損傷時表達上調(diào)的標志物。在腎臟疾病中，其背景與早期腎損傷密切相關(guān)。研究機制上，Kim-1可促進巨噬細胞極化、調(diào)節(jié)炎癥反應，還參與腎小管上皮細胞修復與再生過程，是腎損傷研究的重要靶點。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，科研人員可將其用于犬類腎臟疾病的機制研究，例如急性腎損傷模型的生物標志物動態(tài)監(jiān)測、藥物或化學物質(zhì)腎毒性評估，以及慢性腎病進展的相關(guān)性分析。兼容組織勻漿樣本的特性支持離體腎臟組織的蛋白表達研究，而血清和血漿的檢測能力則為體內(nèi)樣本的實時分析提供便利，尤其適用于縱向研究中多次采樣數(shù)據(jù)的對比。是探索犬類腎臟病理生理機制及治療干預效果的重要工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    Kim-1
  • 種屬：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/ml.
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of canine Kim-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    91

    Range %

    86-95

    1:2

    Average %

    102

    Range %

    97-107

    1:4

    Average %

    91

    Range %

    85-97

    1:8

    Average %

    97

    Range %

    91-103

  • 回收率：

    The recovery of canine Kim-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    87

    80-93

    EDTA plasma (n=4)

    90

    83-96

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    20

    2.449

    2.563

    2.506

    2.348

    10

    1.595

    1.538

    1.567

    1.409

    5

    1.001

    1.052

    1.027

    0.869

    2.5

    0.687

    0.648

    0.668

    0.510

    1.25

    0.463

    0.473

    0.468

    0.310

    0.625

    0.306

    0.314

    0.310

    0.152

    0.312

    0.221

    0.232

    0.227

    0.069

    0

    0.154

    0.161

    0.158

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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