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  4. Chicken Immunoglobulin M(IgM)ELISA Kit

Chicken Immunoglobulin M(IgM)ELISA Kit

  • 中文名稱：
    雞免疫球蛋白M(IgM)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E16200C
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    雞免疫球蛋白M(IgM)酶聯(lián)免疫試劑盒（CSB-E16200C）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的IgM含量。IgM是一種在體液免疫和黏膜免疫中發(fā)揮重要作用的免疫球蛋白，具有10或12個抗原結(jié)合位點(diǎn)，能夠以高親和力結(jié)合抗原靶標(biāo)。其研究機(jī)制涉及IgM五聚體的組裝、與黏膜轉(zhuǎn)運(yùn)受體pIgR的相互作用以及與特異性受體FcμR的結(jié)合等。試劑盒檢測范圍為7.8 ng/mL-2000 ng/mL，科研人員可將其應(yīng)用于家禽免疫機(jī)制研究、疫苗效力評估、傳染病模型建立等場景，例如在禽流感疫苗開發(fā)中監(jiān)測免疫雞群IgM水平變化，或在沙門氏菌感染實(shí)驗(yàn)中分析抗體應(yīng)答動態(tài)，為禽類免疫學(xué)研究提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    IgM
  • 種屬：
    Gallus gallus (Chicken)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    7.8 ng/mL-2000 ng/mL
  • 靈敏度：
    7.8 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1000Average %92
    Range %82-96
    1:2000Average %94
    Range %91-97
    1:4000Average %98
    Range %92-104
    1:8000Average %92
    Range %87-98
  • 回收率：
    The recovery of chicken IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9791-103
    EDTA plasma (n=4)8682-91
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2Average
    20000.343 0.345 0.344
    5000.554 0.575 0.565
    1250.827 0.856 0.842
    31.251.122 1.158 1.140
    7.811.267 1.367 1.317
    01.764 1.881 1.823
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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