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Pig Immunoglobulin M,IgM ELISA Kit

  • 中文名稱:
    豬免疫球蛋白M(IgM)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E06805p
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3800/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    豬免疫球蛋白M(IgM)酶聯(lián)免疫試劑盒(CSB-E06805p)為競爭法ELISA試劑盒,定量檢測血清、血漿樣本中的IgM含量。IgM是人體最早產(chǎn)生的免疫球蛋白,在抗感染早期發(fā)揮重要作用,可高效激活補體系統(tǒng)。其作為靶點的研究機制主要是基于它與疾病的關聯(lián),通過設計藥物或抗體與之結合,調節(jié)免疫反應,為自身免疫病、感染性疾病等的治療打開新思路。試劑盒檢測范圍為0.039 μg/mL-10 μg/mL,常用于科研領域評估疫苗效果、感染性疾病模型構建或免疫機制研究;可為豬類免疫相關研究提供可靠數(shù)據(jù)支持,例如傳染病防控、免疫增強劑開發(fā)或營養(yǎng)干預實驗中的免疫指標監(jiān)測本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫:
    IgM
  • 種屬:
    Sus scrofa (Pig)
  • 樣本類型:
    serum, plasma
  • 檢測范圍:
    0.039 μg/mL-10 μg/mL
  • 靈敏度:
    0.039 μg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 97  
    Range % 92-103  
    1:2 Average % 87  
    Range % 81-92  
    1:4 Average % 95  
    Range % 90-99  
    1:8 Average % 102  
    Range % 96-108  
  • 回收率:
    The recovery of pig IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 91-102  
    EDTA plasma (n=4) 104 98-108  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average    
    10 0.204 0.206 0.205    
    2.5 0.463 0.454 0.459    
    0.62 0.915 0.936 0.926    
    0.16 1.429 1.488 1.459    
    0.04 2.056 2.134 2.095    
    0 2.515 2.652 2.584    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

引用文獻

產(chǎn)品評價

平均分:
5.0分 - 1 個評價

樣品類型:血清

樣品信息:其他 豬

稀釋比:沒有稀釋

產(chǎn)品評價: 標曲準確,樣本數(shù)值在標曲范圍之內,符合預期結果。

By 謝老師