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Chicken ovalbumin ELISA Kit

  • 中文名稱:
    雞卵清白蛋白酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E13315C
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥4200/¥3000
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    雞卵清白蛋白酶聯(lián)免疫試劑盒(CSB-E13315C)為競爭法ELISA試劑盒,定量檢測血清、血漿樣本中的OVA含量。OVA即卵清蛋白,常作為抗原用于免疫學(xué)研究。其背景上,它來源廣泛、性質(zhì)穩(wěn)定。研究機(jī)制方面,它可刺激機(jī)體產(chǎn)生免疫反應(yīng),常被用于構(gòu)建免疫相關(guān)疾病模型,以研究免疫細(xì)胞活化、免疫應(yīng)答過程和免疫調(diào)節(jié)機(jī)制等。試劑盒檢測范圍為3.125 μg/mL-400 μg/mL,科研人員可將其用于疫苗開發(fā)中的抗原定量、過敏反應(yīng)模型建立、免疫復(fù)合物形成機(jī)制研究等領(lǐng)域,特別適用于禽類疫苗研發(fā)、食物過敏原檢測或免疫應(yīng)答動(dòng)態(tài)監(jiān)測等實(shí)驗(yàn)場景本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    Allergen Gal d II ELISA Kit; Egg albumin ELISA Kit; OVAL_CHICK ELISA Kit; Ovalbumin ELISA Kit; Plakalbumin ELISA Kit; Serpin peptidase inhibitor ELISA Kit; SERPINB14 ELISA Kit
  • 縮寫:
    OVA
  • Uniprot No.:
  • 種屬:
    Gallus gallus (Chicken)
  • 樣本類型:
    serum, plasma
  • 檢測范圍:
    3.125 μg/mL-400 μg/mL
  • 靈敏度:
    3.125 μg/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Cancer
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 標(biāo)準(zhǔn)曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    μg/ml

    OD1

    OD2

    Average

    400

    0.178

    0.169

    0.174

    200

    0.313

    0.327

    0.320

    50

    0.623

    0.628

    0.625

    12.5

    1.146

    1.129

    1.138

    3.125

    1.753

    1.721

    1.737

    0

    2.500

    2.503

    2.502

  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能:
    Non-inhibitory serpin. Storage protein of egg white.
  • 基因功能參考文獻(xiàn):
    1. 120A and C30/73/120A mutants formed no SS form, in contrast to C73/120A as a control. Thus, we concluded that Cys30 participates in the correct folding of OVA, and that its SS bond (Cys11-Cys30) is transiently generated during the early folding stage to avoid misfolding, and then the native SS form of OVA is regenerated through SH-SS exchanges. PMID: 29175210
    2. Data show that both ovalbumin and ovomucoid were efficiently mutagenized in cultured primordial germ cells (PGCs) by CRISPR/Cas9 system. PMID: 27050479
    3. Data show that both green and red light wavelengths induce structural changes in ovalbumin. PMID: 26656181
    4. found that ovalbumin packs into a bicontinuous structure with a significant void space and that ovalbumin is highly ordered within the dense network PMID: 26488663
    5. Cluster analysis revealed a first group of proteins related to mineralization and mainly present at the onset of calcification including OVOT, OVAL, OC-17, and two novel calcium binding proteins (EDIL3, MFGE8). PMID: 25284052
    6. Secondary and tertiary structures of OVA were characterised to show the relationship between potential allergenicity and the conformation of OVA after heating and glycation. PMID: 23915026
    7. Inhalation tolerance limits aeroallergen OVA peptide capture by airway mucosal dendritic cell subsets via a mechanism of bone marrow suppression of dendritic cell precursor function coupled with reduced antigen availability in vivo at the airway mucosa. PMID: 21930961
    8. Lysozyme (pI = 9.3) destabilizes the liquid amorphous calcium carbonate emulsion whereas the glycoprotein ovalbumin (pI = 4.7) extends the lifespan of the emulsified state remarkably. PMID: 21736300
    9. Fibrosarcoma-secreting vesicle-bound ovalbumin elicits a strong antigen-specific CD8-type T cell response, CD4-positive T cell help, antigen-specific antibodies, and a decrease in the percentage of immunosuppressive regulatory T cells in the tumor. PMID: 21705625
    10. The presence of the original SS bridge in ovalbumin contributes to conformational stability but is not directly related to the conversion to heat-stable ovalbumin (S-OVA). PMID: 21389617
    11. These authors report the kinetics for the rate of acetylation during protein synthesis using ovalabumin. PMID: 272676
    12. Reports details of the organization of the ovalbumin gene exon-intron structure. PMID: 283395
    13. Authors measure the kinetics of mRNA binding to microsomes during translation & nascent chain elongation rates. PMID: 6749856
    14. Demonstrates the kinetics of I-ovalbumin conformer serine peptidase inhibition. PMID: 8939987
    15. Demonstrates that the I-ovalabumin conformer binds bovine trypsin PMID: 15488772
    16. Results provide a detailed equilibrium and kinetic characterization of the interaction between the serpin ovalbumin and bovine trypsin. PMID: 15488772
    17. the carbohydrate chain and two phosphate moieties do not affect hydrophobic collapse in the kinetic refolding of hen ovalbumin but play an important role in the slow rearrangement, by blocking the off-pathway reaction that competes with correct refolding PMID: 15618616
    18. Chromatin immunoprecipitation assays showed that the ovalbumin control region was associated with acetylated histone H3 but not with dimethylated histone H3 at Lys 27. PMID: 16794325
    19. Intracerebral, but not systemic, infection with attenuated Listeria-secreting ovalbumin induces a neurological model syndrome in transgenic neuron-specific enolase-OVA mice after brain bacterial clearance, whereas wild-type mice remain healthy. PMID: 18523310

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  • 亞細(xì)胞定位:
    Secreted.
  • 蛋白家族:
    Serpin family, Ov-serpin subfamily
  • 組織特異性:
    Major protein of egg white. Expressed in the magnum of the oviduct (at protein level).
  • 數(shù)據(jù)庫鏈接:

    KEGG: gga:396058

    STRING: 9031.ENSGALP00000036403

    UniGene: Gga.623