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  4. Dog C-C motif chemokine 5(CCL5) ELISA kit

Dog C-C motif chemokine 5(CCL5) ELISA kit

  • 中文名稱：
    犬CC趨化因子5(CCL5)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL004800DO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產品詳情

  • 產品描述：
    犬CC趨化因子5(CCL5)酶聯(lián)免疫試劑盒（CSB-EL004800DO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CCL5含量。CCL5是一種趨化因子，在免疫調節(jié)、炎癥反應等生理和病理過程中發(fā)揮重要作用。其研究機制主要圍繞與受體結合，引導免疫細胞遷移和活化，參與腫瘤微環(huán)境形成、炎癥性疾病發(fā)展等，在抗腫瘤、抗炎等治療領域有潛在應用價值。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，靈敏度為0.081 ng/mL。該產品適用于實驗動物模型研究，如評估犬類炎癥性疾病進程、免疫相關病理機制探索或藥物干預實驗中趨化因子動態(tài)變化的定量監(jiān)測，為獸醫(yī)學、比較醫(yī)學及免疫學領域提供可靠的檢測工具。本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
  • 別名：
    CCL5 ELISA kit; SCYA5C-C motif chemokine 5 ELISA kit; Small-inducible cytokine A5 ELISA kit; T-cell-specific protein RANTES ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.081 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of dog CCL5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 105    
    Range % 97-110    
    1:2 Average % 101    
    Range % 95-106    
    1:4 Average % 96    
    Range % 87-101    
    1:8 Average % 102    
    Range % 97-107    
  • 回收率：
    The recovery of dog CCL5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 104 96-108    
    EDTA plasma (n=4) 99 91-104    
                   
                   
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected   CV
    10 2.658 2.600 2.629 2.510   1.56%
    5 1.985 2.016 2.001 1.882   1.10%
    2.5 1.348 1.369 1.359 1.240   1.09%
    1.25 0.919 0.927 0.923 0.804   0.61%
    0.625 0.502 0.523 0.513 0.394   2.89%
    0.313 0.294 0.314 0.304 0.185   4.65%
    0.156 0.212 0.219 0.216 0.097   2.29%
    0 0.117 0.121 0.119 　   2.38%
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 最新研究進展：
        CCL5（C-C motif ligand 5），也稱為RANTES（Regulated on Activation, Normal T Expressed and Secreted），是一種由多種細胞產生的細胞因子，包括T細胞、巨噬細胞和上皮細胞。CCL5在多種疾病中發(fā)揮重要作用，如炎癥、艾滋病毒感染、自身免疫疾病和腫瘤。最近的研究表明，CCL5不僅在炎癥和免疫過程中發(fā)揮作用，還可以促進腫瘤生長、侵襲和轉移。
     
  • 功能：
    Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.
  • 基因功能參考文獻：
    1. Data indicate that the Leishmania braziliensis antigens plus saponin (LBSap) vaccine induced high levels of IL-12, IL-10, CCL4, CCL5 and CXCL8 expression within 48 hours. PMID: 23911411
    2. Adipose tissue-derived mesenchymal stem cells expressed the mRNA of transforming growth factor beta (TGF-beta), IL-6, IL-8, CCL2, CCL5, VEGF,HGF, tissue inhibitor metalloproteinase-1/2, and cyclooxygenase-2 but not that of IL-10. PMID: 18717642
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 數(shù)據(jù)庫鏈接：

    KEGG: cfa:403522

    STRING: 9615.ENSCAFP00000026835

    UniGene: Cfa.21