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  4. Dog Complement 3(C3)ELISA Kit

Dog Complement 3(C3)ELISA Kit

  • 中文名稱：
    犬補(bǔ)體蛋白3(C3)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL003888DO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    犬補(bǔ)體蛋白3(C3)酶聯(lián)免疫試劑盒（CSB-EL003888DO）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的C3含量。C3是一種重要靶點(diǎn)。它在補(bǔ)體系統(tǒng)中扮演關(guān)鍵角色，參與免疫防御等生理過程。研究發(fā)現(xiàn)其異常激活與多種疾病相關(guān)，如自身免疫病、腎臟疾病等?？蒲腥藛T探究其激活機(jī)制，期望通過干預(yù)C3來開發(fā)新的治療手段。試劑盒檢測范圍為0.78 μg/mL-50 μg/mL，本試劑盒適用于實(shí)驗(yàn)動物模型研究、免疫病理機(jī)制探索及藥物干預(yù)效果評價(jià)等科研場景，例如通過檢測感染性疾病或免疫相關(guān)疾病模型中C3的濃度變化，揭示補(bǔ)體系統(tǒng)在犬類疾病發(fā)生發(fā)展中的作用，為獸醫(yī)學(xué)研究提供可靠的數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    AHUS5, ARMD9, ASP, CPAMD1, acylation-stimulating protein cleavage product|complement component C3
  • 縮寫：
  • 種屬：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.78 μg/mL-50 μg/mL
  • 靈敏度：
    0.195 μg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of dog C3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:100Average %99
    Range %95-102
    1:200Average %95
    Range %81-89
    1:400Average %92
    Range %88-96
    1:800Average %101
    Range %98-103
  • 回收率：
    The recovery of dog C3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9491-97
    EDTA plasma (n=4)8985-93
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/mlOD1OD2Average
    500.075 0.079 0.077
    250.130 0.136 0.133
    12.50.206 0.204 0.205
    6.250.322 0.332 0.327
    3.120.543 0.538 0.541
    1.560.851 0.878 0.865
    0.781.163 1.198 1.181
    02.014 2.036 2.025
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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