Dog angiotensin Ⅱ (ANG-Ⅱ) ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

犬血管緊張素Ⅱ(ANG-Ⅱ)酶聯(lián)免疫試劑盒（CSB-E15778c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的ANG-Ⅱ含量。ANG - Ⅱ即血管緊張素Ⅱ，是腎素 - 血管緊張素系統(tǒng)的關(guān)鍵效應(yīng)肽。它在血壓調(diào)節(jié)、水電解質(zhì)平衡等方面發(fā)揮重要作用。研究機制上，主要通過與特異性受體結(jié)合，激活一系列細(xì)胞內(nèi)信號通路，引發(fā)血管收縮、醛固酮釋放等生理反應(yīng)。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，適用于科研場景下評估藥物干預(yù)、疾病模型或生理狀態(tài)對ANG - Ⅱ水平的影響，例如犬類高血壓機制探索、腎臟疾病相關(guān)生物標(biāo)志物分析或激素調(diào)控研究，為獸醫(yī)科研及動物模型開發(fā)提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

angiotension Ⅱ, angiotensin 2, ANG2
縮寫：

ANG-Ⅱ
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma
檢測范圍：

6.25 pg/mL-400 pg/mL
靈敏度：

1.56 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog ANG-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-93
1:2	Average %	98
	Range %	92-104
1:4	Average %	88
	Range %	86-93
1:8	Average %	89
	Range %	84-96

回收率：

The recovery of dog ANG-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-97
EDTA plasma (n=4)	100	95-102

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.