Rat angiotensin II (ANG-Ⅱ) ELISA kit
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中文名稱:大鼠血管緊張素Ⅱ(ANG-Ⅱ)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E04494r
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規(guī)格:96T/48T
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價(jià)格:¥3900/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:大鼠血管緊張素Ⅱ(ANG-Ⅱ)酶聯(lián)免疫試劑盒(CSB-E04494r)為競(jìng)爭(zhēng)法ELISA試劑盒,定量檢測(cè)血清、血漿.樣本中的ANG-Ⅱ含量。ANG-Ⅱ是重要的靶點(diǎn)。它是腎素-血管緊張素系統(tǒng)的關(guān)鍵效應(yīng)肽。其作用機(jī)制主要是通過(guò)與受體結(jié)合,收縮血管、促進(jìn)醛固酮分泌,影響血壓調(diào)節(jié)、水鹽平衡等。對(duì)它的研究有助于深入了解心血管疾病等病理生理過(guò)程,為相關(guān)治療提供方向。試劑盒檢測(cè)范圍為15.6 pg/ml-1000 pg/ml.,該產(chǎn)品適用于基礎(chǔ)科研場(chǎng)景,如心血管疾病動(dòng)物模型研究、藥物干預(yù)對(duì)血管活性物質(zhì)的影響評(píng)估,或腎臟功能與體液平衡相關(guān)機(jī)制探索,為實(shí)驗(yàn)室提供穩(wěn)定可靠的檢測(cè)工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
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別名:angiotensin II,ANG-Ⅱ,angiotensin 2,ANG-2
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縮寫:ANG-Ⅱ
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種屬:Rattus norvegicus (Rat)
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樣本類型:serum, plasma.
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檢測(cè)范圍:15.6 pg/ml-1000 pg/ml.
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靈敏度:7.8 pg/ml.
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Cardiovascular
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測(cè)定原理:quantitative
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測(cè)定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<12%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat ANG-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
84
Range %
80-92
1:2
Average %
87
Range %
81-95
1:4
Average %
90
Range %
92-97
1:8
Average %
93
Range %
86-98
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回收率:
The recovery of rat ANG-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
92
86-98
EDTA plasma (n=4)
87
82-95
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標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
1000
0.158
0.160
0.159
500
0.185
0.189
0.187
250
0.237
0.232
0.235
125
0.364
0.340
0.352
62.5
0.595
0.568
0.581
31.25
0.958
0.902
0.930
15.6
1.355
1.298
1.326
0
2.287
2.126
2.206
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數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Therapeutic Effects of Total Secondary Ginsenosides on Chronic Heart Failure in Rats and Dogs: A Comparative Study with Xinbao Pills X Xu, Y Zhang, Y Xiao, A Zhang,Natural Product Communications,2025
- Quercetin prophylaxis protects the kidneys by modulating the renin–angiotensin–aldosterone axis under acute hypobaric hypoxic stress V Rathi,Scientific reports,2024
- Panax notoginseng saponin alleviates pulmonary fibrosis in rats by modulating the renin-angiotensin system H Li,Journal of ethnopharmacology,2024
- Chicken Muscle-Derived ACE2 Upregulating Peptide VVHPKESF Inhibits Angiotensin II-Stimulated Inflammation in Vascular Smooth Muscle Cells via the ACE2/Ang (1–7)/MasR Axis H Fan,Journal of agricultural and food chemistry,2023
- Erythropoietin alleviates post-resuscitation myocardial dysfunction in rats potentially through increasing the expression of angiotensin II receptor type 2 in myocardial tissues Zhou H,Molecular medicine reports,2023
- Effects of Exercise Preconditioning on Doxorubicin-Induced Liver and Kidney Toxicity in Male and Female Rats FP Boeno,International journal of molecular sciences,2023
- Panax Notoginseng Saponin Alleviates Pulmonary Fibrosis in Rats by Modulating the Renin-Angiotensin System H Li,Available at SSRN 4464436,2023
- Biomimetic Nanoparticle-Mediated Target Delivery of Hypoxia-Responsive Plasmid of Angiotensin-Converting Enzyme 2 to Reverse Hypoxic Pulmonary Hypertension R Yuan,ACS nano,2023
- Effects of Long-Term Intervention with Losartan, Aspirin and Atorvastatin on Vascular Remodeling in Juvenile Spontaneously Hypertensive Rats Q Liu,Molecules,2023
- Effects of intrarenal angiotensin 1–7 infusion on renal haemodynamic and excretory function in anaesthetised two-kidney one-clip and deoxycorticosterone acetate-salt hypertensive rats EF Barry,Experimental physiology,2022
- Natural sea salt in diet ameliorates better protection compared to table salt in the doxorubicin-induced cardiac remodeling F Anwar,Journal of Taibah University for Science,2022
- FACTORES DE PREDISPOSICIóN AL DA?O RENAL AGUDO PRODUCIDO POR LOS ANTIINFLAMATORIOS NO ESTEROIDEOS OA Hidalgo Thomas,TESIS DOCTORAL,2022
- Candesartan Cilexetil Attenuates Arrhythmogenicity Following Pressure Overload in Rats via the Modulation of Cardiac Electrical and Structural Remodeling and Calcium Handling Dysfunction GJ Chang,Journal of the American Heart Association,2022
- Improving effects of eplerenone on atrial remodeling induced by chronic intermittent hypoxia in rats. Y Yang,Cardiovascular pathology,2022
- Activation of angiotensin-converting enzyme 2/angiotensin (1–7)/mas receptor axis triggers autophagy and suppresses microglia proinflammatory polarization via forkhead box class O1 signaling R Dang,Aging cell,2021
- IAI CONFERENCE: The influence of extracts and fractions from matoa leaves (Pometia pinnata) on angiotensin I levels Ika Purwidyaningrum,Pharmacy Education,2021
- Peperomia pellucida (L.) Kunth as an Angiotensin-converting Enzyme Inhibitor in Two-kidney, One-clip Goldblatt Hypertensive Rats FC Saputri,Saudi Journal of Biological Sciences,2021
- Ginsenoside Rg3 Attenuates Early Hepatic Injury via Inhibiting PPARγ-and Ang II-Related Inflammation and Fibrosis in Type II Diabetic Mice Y Jiang,Natural Product Communications,2021
- CoQ10 augments candesartan protective effect against tourniquet-induced hind limb ischemia-reperfusion: involvement of non-classical RAS and ROS pathways AS Awad,Saudi Pharmaceutical Journal,2021
- Endogenous β-endorphin Plays a Pivotal Role in Angiotensin II-mediated Central Neurochemical Changes and Pressor Response SK Badr,Chemico-Biological Interactions,2021
- Quercetin prevents myocardial infarction adverse remodeling in rats by attenuating TGF-β1/Smad3 signaling: different mechanisms of action GM Albadrani,Saudi Journal of Biological Sciences,2021
- Effects of carotid baroreceptor stimulation on aortic remodeling in obese rats Q Yu,Nutrition, Metabolism and Cardiovascular Diseases,2021
- Vitamin A deficiency indicating as low expression of LRAT may be a novel biomarker of primary hypertension X Liang,Journal Clinical and Experimental Hypertension,2020
- Irradiation of carotid baroreceptor with low‐intensity pulsed ultrasound exerts different metabolic protection in perirenal, epididymal white adipose tissue and interscapular brown adipose tissue of obese rats Q Cao,FASEB J,2020
- Increased angiotensin II from adipose tissue modulates myocardial collagen I and III in obese rats DCT da Silva-Bertani,Life Sciences,2020
- Low-intensity focused ultrasound stimulation treatment decreases blood pressure in spontaneously hypertensive rats D Li,IEEE Transactions on Biomedical Engineering,2020
- Probiotics Mitigates Skeletal Muscle Atrophy in Isoproterenol-Induced Heart Failure Adult Rats; by Increasing IGF-I Gene Expression and Improving Insulin … MS ABD-EL HAMID,medicaljournalofcairouniversity,2020
- In vivo safety profile of a CSPG4-directed IgE antibody in an immunocompetent rat model Williams I P, et al,/,2019
- Ginsenoside Rg3 Attenuates Angiotensin II-Mediated Renal Injury in Rats and Mice by Upregulating Angiotensin-Converting Enzyme 2 in the Renal Tissue Liu H, et al,Evidence-Based Complementary and Alternative Medicine,2019
- Probiotics Mitigates Skeletal Muscle Atrophy in Isoproterenol-Induced Heart Failure Adult Rats; by Increasing IGF-I Gene Expression and Improving Insulin Sensitivity MOHAMED A B D E L H A, et al,The Medical Journal of Cairo University,2019
- Physical training prior to myocardial infarction potentializes stem cell therapy, SDF-1/CXCR4 axis activation and inhibits the vasoconstrictor response in hypertensive rats Maximiliano I.Schaun, et al,Cytokine,2019
- Administration of losartan preserves cardiomyocyte size and prevents myocardial dysfunction in tail-suspended mice by inhibiting p47phox phosphorylation, NADPH oxidase activation and MuRF1 expression Liang L, et al,Journal of Translational Medicine,2019
- Negative regulation of eNOS-NO signaling by over-SUMOylation of PPARγ contributes to insulin resistance and dysfunction of vascular endothelium in rats WanwanYuan, et al,Journal of Cardiovascular Pharmacology,2019
- Dynamic Variation of RAS on Silicotic Fibrosis Pathogenesis in Rats Zhang BN, et al,Current Medical Science,2019
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靶點(diǎn)詳情
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最新研究進(jìn)展:ANG-Ⅱ是一種細(xì)胞因子,在機(jī)體內(nèi)具有廣泛的生物學(xué)功能,包括調(diào)節(jié)血壓、腎功能和心血管系統(tǒng)等。近年來(lái)的研究發(fā)現(xiàn),ANG-Ⅱ還具有免疫調(diào)節(jié)作用,能夠通過(guò)調(diào)節(jié)細(xì)胞凋亡、自噬、炎癥反應(yīng)等多種機(jī)制調(diào)節(jié)機(jī)體的免疫反應(yīng)。
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