Fish Complement 3,C3 ELISA Kit
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中文名稱:魚補(bǔ)體蛋白3(C3)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E09727s
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規(guī)格:96T/48T
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價(jià)格:¥4200/¥3000
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:魚補(bǔ)體蛋白3(C3)酶聯(lián)免疫試劑盒(CSB-E09727s)為競(jìng)爭(zhēng)法ELISA試劑盒,定量檢測(cè)血清、血漿樣本中的C3含量。C3是補(bǔ)體系統(tǒng)重要成分。其背景在于參與免疫防御、免疫自穩(wěn)等。研究機(jī)制方面,它可被多種途徑激活,裂解為多個(gè)片段,參與調(diào)理吞噬、介導(dǎo)炎癥反應(yīng)等,在抗感染、自身免疫病等研究中有重要價(jià)值。試劑盒檢測(cè)范圍為12.5 μg/mL-500 μg/mL,該產(chǎn)品廣泛應(yīng)用于水產(chǎn)動(dòng)物免疫機(jī)制研究,如細(xì)菌或病毒感染模型中的補(bǔ)體通路分析、養(yǎng)殖環(huán)境壓力對(duì)免疫功能的評(píng)估,以及疫苗或免疫增強(qiáng)劑的功效驗(yàn)證等實(shí)驗(yàn)場(chǎng)景,為水生生物健康管理和疾病防控研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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縮寫:
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種屬:Fish
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樣本類型:serum, plasma
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檢測(cè)范圍:12.5 μg/mL-500 μg/mL
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靈敏度:12.5 μg/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長:450 nm
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研究領(lǐng)域:Cancer
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測(cè)定原理:quantitative
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測(cè)定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of fish C3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 85-100 1:2 Average % 96 Range % 90-108 1:4 Average % 95 Range % 91-98 1:8 Average % 95 Range % 91-100 -
回收率:
The recovery of fish C3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 89 85-94 EDTA plasma (n=4) 95 91-102 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. μg/ml OD1 OD2 Average 500 0.176 0.174 0.175 250 0.381 0.393 0.387 115 0.557 0.569 0.563 37.5 0.894 0.901 0.898 12.5 1.481 1.498 1.490 0 2.417 2.542 2.480 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- The Effect of Oxytetracycline on the Immune Response and Expression of Immune‐Related Genes in European Sea Bass (Dicentrarchus labrax, L. 1758) C? Kurtbo?an, E Baba, MD Demircan,Aquaculture Research,2025
- Formulation and Field Evaluation of Palm Oil Adjuvanted Feed-Based Streptococcosis Vaccine in Cage-Cultured Red Hybrid Tilapia. MS Mohammad Ridzuan, A Abdullah,Aquaculture Studies (AquaSt),2025
- Nano-Bacillus amyloliquefaciens as a dietary intervention in nile tilapia (Oreochromis niloticus): Effects on resistance to Aeromonas hydrophila challenge, immune-antioxidant responses, digestive/absorptive capacity, and growth ME Hassanin, A El-Murr, AR El-Khattib, AA Abdelwarith,Heliyon,2024
- Toxicological insight of metiram: immuno-oxidative, neuro-behavioral, and hemato-biochemical changes during acute exposure of Nile tilapia (Oreochromis niloticus) M Shaalan,BMC veterinary research,2024
- Role of Silver Nanoparticles Synthetized by Marine Red Algae, Acanthophora spicifera, Against Saprolegniosis in Oreochromis niloticus M Sayed,/,2023
- Dietary Artemisia arborescens Supplementation Effects on Growth, Oxidative Status, and Immunity of Gilthead Seabream (Sparus aurata L.) OP Tzortzatos,Animals,2024
- Pseudomonas putida infection induces immune-antioxidant, hepato-renal, ethological, and histopathological/immunohistochemical disruptions in Oreochromis niloticus: the palliative role of titanium dioxide nanogel ANA Rahman,BMC veterinary research,2024
- Potential role of dietary white poplar (Populus alba L.) in stimulating growth, digestion, and antioxidant/immune status of Nile tilapia (Oreochromis niloticus) RE Ibrahim,/,2024
- The crosstalk between photoperiod and early mild stress on juvenile oscar (Astronotus ocellatus) after acute stress M Zare,Fish physiology and biochemistry,2024
- Effect of dietary intervention with Capsicum annuum extract on growth performance, physiological status, innate immune response, and related gene expression in Nile tilapia RE Ibrahim,Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology,2023
- The Recovery Time between Early Mild Stress and Final Acute Stress Affects Survival Rate, Immunity, Health, and Physiology of Oscar (Astronotus ocellatus) M Zare,Animals,2023
- Fish Meal Replacement and Early Mild Stress Improve Stress Responsiveness and Survival of Fish after Acute Stress M Zare,Animals,2023
- Early Mild Stress along with Lipid Improves the Stress Responsiveness of Oscar (Astronotus ocellatus) M Esmaeili,Aquaculture Nutrition,2022
- Replacement of dietary fish oil with plant oils improves the immunological responses and the antioxidant status in Oreochromis niloticus exposed to suboptimal temperature RM Reda,Fish Physiology and Biochemistry,2020
- Optimal form of yeast cell wall promotes growth, immunity and disease resistance in gibel carp (Carassius auratus gibelio) P Zhang,Aquaculture Reports,2020
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