Fish Progesterone(PROG) ELISA Kit
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中文名稱:魚孕激素/孕酮(PROG)酶聯(lián)免疫試劑盒
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貨號:CSB-E17295Fh
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規(guī)格:96T/48T
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價格:¥4200/¥3000
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:魚孕激素/孕酮(PROG)酶聯(lián)免疫試劑盒(CSB-E17295Fh)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿.樣本中的PROG含量。靶點(diǎn)研究是藥物研發(fā)的關(guān)鍵,涉及尋找疾病相關(guān)分子或細(xì)胞結(jié)構(gòu)作為治療目標(biāo)。研究機(jī)制包括基因組學(xué)、蛋白質(zhì)組學(xué)和生物信息學(xué)分析,以及高通量篩選技術(shù)。靶點(diǎn)發(fā)現(xiàn)有助于開發(fā)更精確的藥物,提高治療效果。試劑盒檢測范圍為0.15 ng/ml-70 ng/ml,靈敏度為0.2 ng/ml.。本產(chǎn)品適用于科研場景中魚類生殖周期監(jiān)測、環(huán)境內(nèi)分泌干擾物效應(yīng)評估、水產(chǎn)養(yǎng)殖繁殖管理優(yōu)化等研究,兼容多種生物樣本類型,操作便捷且重復(fù)性良好,為實驗室開展魚類激素相關(guān)機(jī)制研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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縮寫:PROG
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種屬:Fish
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樣本類型:serum, plasma, tissue homogenates.
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檢測范圍:0.15 ng/ml-70 ng/ml
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靈敏度:0.2 ng/ml.
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Signal Transduction
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測定原理:quantitative
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測定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of fish PROG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
94
Range %
85-106
1:2
Average %
90
Range %
89-99
1:4
Average %
95
Range %
85-103
1:8
Average %
90
Range %
87-103
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回收率:
The recovery of fish PROG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
95
82-107
EDTA plasma (n=4)
96
88-102
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標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml
OD1
OD2
Average
70
0.143
0.148
0.146
20
0.279
0.264
0.272
5
0.523
0.545
0.534
1
0.861
0.893
0.877
0.15
1.428
1.459
1.444
0
2.113
2.132
2.123
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數(shù)據(jù)處理:
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貨期:3-5 working days
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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最新研究進(jìn)展:PROG是孕酮受體的激動劑,可調(diào)節(jié)生殖系統(tǒng)、乳房和神經(jīng)系統(tǒng)功能。近年來,PROG在抗腫瘤治療和神經(jīng)保護(hù)方面的作用受到了廣泛關(guān)注。研究表明,PROG可通過調(diào)節(jié)細(xì)胞周期、促進(jìn)細(xì)胞凋亡、抑制血管生成等多種途徑發(fā)揮抗腫瘤作用。此外,PROG也能夠保護(hù)神經(jīng)元、促進(jìn)神經(jīng)元再生,并減輕神經(jīng)退行性疾病的癥狀。
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