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  4. Fish adrenocorticotropic hormone (ACTH) ELISA kit

Fish adrenocorticotropic hormone (ACTH) ELISA kit

  • 中文名稱：
    魚(yú)促腎上腺皮質(zhì)激素(ACTH)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E15926Fh
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    魚(yú)促腎上腺皮質(zhì)激素(ACTH)酶聯(lián)免疫試劑盒（CSB-E15926Fh）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的ACTH含量。促腎上腺皮質(zhì)激素（ACTH）由腺垂體分泌，能促進(jìn)腎上腺皮質(zhì)增生及腎上腺皮質(zhì)激素合成與釋放。其研究機(jī)制主要聚焦于調(diào)控糖皮質(zhì)激素分泌、參與應(yīng)激反應(yīng)等。深入研究它能助力理解內(nèi)分泌系統(tǒng)運(yùn)行，為相關(guān)疾病治療提供新思路。試劑盒檢測(cè)范圍為75 pg/mL-1200 pg/mL，對(duì)研究魚(yú)類內(nèi)分泌調(diào)節(jié)機(jī)制、環(huán)境應(yīng)激適應(yīng)性及養(yǎng)殖健康管理具有重要意義；支持科研人員開(kāi)展魚(yú)類生理狀態(tài)評(píng)估、水環(huán)境毒理學(xué)研究及水產(chǎn)養(yǎng)殖中的激素水平監(jiān)測(cè)；為探究魚(yú)類神經(jīng)內(nèi)分泌系統(tǒng)功能、繁殖調(diào)控及應(yīng)激病理機(jī)制提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě)：
    ACTH
  • 種屬：
    Fish
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    75 pg/mL-1200 pg/mL
  • 靈敏度：
    75 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 105  
    Range % 94-109  
    1:2 Average % 92  
    Range % 84-97  
    1:4 Average % 101  
    Range % 98-105  
    1:8 Average % 95  
    Range % 84-98  
  • 回收率：
    The recovery of fish ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 82-97  
    EDTA plasma (n=4) 94 90-98  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    1200 0.149 0.152 0.151    
    600 0.300 0.288 0.294    
    300 0.477 0.491 0.484    
    150 0.871 0.889 0.880    
    75 1.835 1.887 1.861    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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