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  4. Pig adrenocorticotropic hormone (ACTH) ELISA kit

Pig adrenocorticotropic hormone (ACTH) ELISA kit

  • 中文名稱：
    豬促腎上腺皮質(zhì)激素(ACTH)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E12944p
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬促腎上腺皮質(zhì)激素(ACTH)酶聯(lián)免疫試劑盒（CSB-E12944p）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的ACTH含量。促腎上腺皮質(zhì)激素（ACTH）由腺垂體分泌，主要作用是促進(jìn)腎上腺皮質(zhì)增生、合成與分泌糖皮質(zhì)激素。在研究機(jī)制上，它通過(guò)與腎上腺皮質(zhì)細(xì)胞膜上受體結(jié)合，激活腺苷酸環(huán)化酶-cAMP-蛋白激酶系統(tǒng)發(fā)揮作用，對(duì)其研究有助于深入了解內(nèi)分泌調(diào)控機(jī)制及相關(guān)疾病。試劑盒檢測(cè)范圍為6.25 pg/mL-100 pg/mL，該產(chǎn)品適用于動(dòng)物生理機(jī)制研究、疾病模型內(nèi)分泌功能評(píng)估或藥物干預(yù)實(shí)驗(yàn)中的激素水平監(jiān)測(cè)，例如探究應(yīng)激條件下ACTH動(dòng)態(tài)變化、腎上腺相關(guān)病理機(jī)制或藥效學(xué)評(píng)價(jià)等科研場(chǎng)景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě)：
    ACTH
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    6.25 pg/mL-100 pg/mL
  • 靈敏度：
    6.25 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 90  
    Range % 86-96  
    1:2 Average % 99  
    Range % 92-104  
    1:4 Average % 84  
    Range % 80-87  
    1:8 Average % 91  
    Range % 83-99  
  • 回收率：
    The recovery of pig ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 88-99  
    EDTA plasma (n=4) 94 91-97  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    100 0.177 0.188 0.183    
    50 0.332 0.344 0.338    
    25 0.631 0.634 0.633    
    12.5 1.278 1.239 1.259    
    6.25 2.356 2.478 2.417    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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