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  4. Goat vitamin B12 (VB12) ELISA kit

Goat vitamin B12 (VB12) ELISA kit

  • 中文名稱(chēng)：
    山羊維生素B12(VB12)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EQ028114GO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    山羊維生素B12(VB12)酶聯(lián)免疫試劑盒（CSB-EQ028114GO）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的VB12含量。VB12是人體必需的維生素之一，在神經(jīng)系統(tǒng)功能維持、DNA合成等方面發(fā)揮關(guān)鍵作用。研究中，其機(jī)制集中在參與細(xì)胞代謝中的甲基轉(zhuǎn)移反應(yīng)和催化同型半胱氨酸轉(zhuǎn)化為甲硫氨酸等過(guò)程，對(duì)貧血、神經(jīng)系統(tǒng)疾病等的防治研究有重要意義。試劑盒檢測(cè)范圍為1.56 pg/mL-100 pg/mL，適用于科研領(lǐng)域中對(duì)山羊VB12代謝機(jī)制的探索，如飼料營(yíng)養(yǎng)評(píng)估、動(dòng)物生理機(jī)能研究或疾病模型中VB12動(dòng)態(tài)變化的分析；組織勻漿檢測(cè)功能為動(dòng)物組織水平研究提供便利；為畜牧業(yè)、獸醫(yī)學(xué)及基礎(chǔ)科研領(lǐng)域的VB12相關(guān)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě)：
    VB12
  • 種屬：
    Capra hircus (Goat)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    1.56 pg/mL-100 pg/mL
  • 靈敏度：
    0.39 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of goat VB12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:10 Average % 85
    Range % 80-92
    1:20 Average % 97
    Range % 90-105
    1:40 Average % 97
    Range % 92-104
    1:80 Average % 92
    Range % 86-98
  • 回收率：
    The recovery of goat VB12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 89-100
    EDTA plasma (n=4) 97 90-100
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average 　
    100 0.145 0.148 0.147 　
    50 0.221 0.227 0.224 　
    25 0.396 0.394 0.395 　
    12.5 0.837 0.815 0.826 　
    6.25 1.252 1.243 1.248 　
    3.12 2.018 2.002 2.010 　
    1.56 2.679 2.641 2.660 　
    0 2.754 2.716 2.735 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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