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Human Vitamin B12,VB12 ELISA Kit

  • 中文名稱(chēng):
    人維生素B12(VB12)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E07903h
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人維生素B12(VB12)酶聯(lián)免疫試劑盒(CSB-E07903h)為競(jìng)爭(zhēng)法ELISA試劑盒,定量檢測(cè)血清、血漿樣本中的VB12含量。VB12即維生素B12,是人體必需的水溶性維生素。它參與神經(jīng)系統(tǒng)發(fā)育、造血等重要生理過(guò)程。研究機(jī)制上,它作為輔酶參與甲基轉(zhuǎn)移反應(yīng)和L-甲基丙二酰輔酶A異構(gòu)化反應(yīng),缺乏VB12會(huì)導(dǎo)致巨幼細(xì)胞貧血、神經(jīng)系統(tǒng)損害等疾病。試劑盒檢測(cè)范圍為1.56 pg/mL-100 pg/mL,靈敏度為1.498 pg/mL。適用于細(xì)胞培養(yǎng)、動(dòng)物模型或生物樣本中的VB12動(dòng)態(tài)監(jiān)測(cè),支持營(yíng)養(yǎng)干預(yù)研究、代謝性疾病機(jī)制探索及藥物開(kāi)發(fā)中的生物標(biāo)志物分析。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě):
    VB12
  • 種屬:
    Homo sapiens (Human)
  • 樣本類(lèi)型:
    serum, plasma
  • 檢測(cè)范圍:
    1.56 pg/mL-100 pg/mL
  • 靈敏度:
    1.498 pg/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Metabolism
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Competitive
  • 精密度:

     

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

     

  • 線(xiàn)性度:

     

    To assess the linearity of the assay, samples were spiked with high concentrations of human VB12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

  • 標(biāo)準(zhǔn)曲線(xiàn):

     

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

     

  • 本試劑盒所含材料:
      • A 96-well Assay plate --The 96-well plate has been pre-coated with anti-human VB12 antibody.
      • Standard (2 x 0.5 ml)--Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
      • Biotin-conjugated VB12(100 x concentrate) (1 x 600 μl)
      • HRP-avidin (100 x concentrate) (1 x 120 μl)
      • Biotin-conjugate Diluent (1 x 10 ml) --Dilute the Biotin-conjugated VB12
      • HRP-avidin Diluent (1 x 20 ml) --Dilute the HRP-avidin solution.
      • Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
      • Wash Buffer (25x concentrate) (1 x 20 ml)--Wash away unbound or free substances.
      • TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • Stop Solution (1 x 10ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells)
      • An Instruction manual

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  • 本試劑盒不含材料:
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

相關(guān)問(wèn)答

 常見(jiàn)問(wèn)題解答
Q:

Protocol P9, step 3 - Set a Blank well without any solution.
This is a completely empty well with no solution or liquid at all? Am I correct?
May I know if this is S0. If it is S0, do we add the wash buffer, TMB and stop solution?

A:
Thanks for your inquiry.
Actually step3 is just only one procedure during the whole experiment. You need to check the full steps according our manual.
This kit is competitive one and the blank is different from S0 indeed. The blank should be added Wash Buffer+TMB Substrate+Stop Solution.
You could see it from the protocol. Pls read the manual carefully.

靶點(diǎn)詳情

  • 最新研究進(jìn)展:
    維生素B12是一種必需營(yíng)養(yǎng)素,可通過(guò)攝入動(dòng)物性食品或補(bǔ)充劑獲得。近年來(lái)的研究表明,維生素B12缺乏與多種慢性疾病如糖尿病、心血管疾病和某些癌癥的發(fā)病率增加有關(guān)。