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  4. Guinea pig interleukin 5,IL-5 ELISA Kit

Guinea pig interleukin 5,IL-5 ELISA Kit

  • 中文名稱：
    豚鼠白介素5(IL-5)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E12064Gp
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豚鼠白介素5(IL-5)酶聯(lián)免疫試劑盒（CSB-E12064Gp）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IL5含量。IL5是重要的治療靶點(diǎn)。它主要由活化的T淋巴細(xì)胞產(chǎn)生，能促進(jìn)嗜酸性粒細(xì)胞的生長(zhǎng)、分化和存活。針對(duì)IL5的研究主要圍繞阻斷其與受體結(jié)合，以減少嗜酸性粒細(xì)胞數(shù)量，進(jìn)而治療哮喘、嗜酸性粒細(xì)胞增多癥等嗜酸性粒細(xì)胞相關(guān)疾病。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL，適用于豚鼠疾病模型的免疫機(jī)制研究，例如哮喘、寄生蟲感染等實(shí)驗(yàn)中的炎癥因子動(dòng)態(tài)監(jiān)測(cè)，或用于評(píng)估免疫調(diào)節(jié)藥物對(duì)IL - 5通路的影響。組織勻漿樣本可用于分析肺、骨髓等靶器官的局部IL - 5表達(dá)水平，為科研人員提供穩(wěn)定可靠的定量數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    IL5Interleukin-5 ELISA Kit; IL-5 ELISA Kit; Eosinophil differentiation factor ELISA Kit; T-cell replacing factor ELISA Kit; TRF ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Guinea Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.81 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %90
    Range %87-96
    1:2Average %94
    Range %89-99
    1:4Average %103
    Range %98-108
    1:8Average %100
    Range %91-105
  • 回收率：
    The recovery of guinea pig IL-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9592-97
    EDTA plasma (n=4)9284-98
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.516 2.489 2.503 2.391
    10001.384 1.356 1.370 1.258
    5000.733 0.698 0.716 0.604
    2500.384 0.375 0.380 0.268
    1250.253 0.234 0.244 0.132
    62.50.187 0.181 0.184 0.072
    31.250.142 0.133 0.138 0.026
    00.113 0.111 0.112 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    IL-5 family
  • 數(shù)據(jù)庫(kù)鏈接：