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  4. Hamster Interleukin-10(IL10) ELISA kit

Hamster Interleukin-10(IL10) ELISA kit

  • 中文名稱：
    倉(cāng)鼠白介素10(IL10)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL011580HA
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    倉(cāng)鼠白介素10(IL10)酶聯(lián)免疫試劑盒（CSB-EL011580HA）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IL10含量。IL10是一種細(xì)胞因子，其背景在于它具有免疫調(diào)節(jié)作用，能抑制炎癥反應(yīng)。研究機(jī)制上，它可作用于多種免疫細(xì)胞，通過與特異性受體結(jié)合，激活下游信號(hào)通路，調(diào)控細(xì)胞因子的產(chǎn)生與釋放，在免疫性疾病、感染等研究領(lǐng)域備受關(guān)注。試劑盒檢測(cè)范圍為6.25 pg/mL-400 pg/mL，IL10水平變化常被用于評(píng)估感染性疾病、自身免疫病等病理模型的免疫狀態(tài)；尤其適用于倉(cāng)鼠炎癥模型研究、疫苗免疫效果評(píng)價(jià)或藥物干預(yù)實(shí)驗(yàn)中的生物標(biāo)志物分析本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    CSIF, IL-10, IL10A, MGC126450, MGC126451, TGIF, cytokine synthesis inhibitory factor
  • 縮寫：
  • 種屬：
    Mesocricetus auratus (Golden hamster)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    6.25 pg/mL-400 pg/mL
  • 靈敏度：
    1.56 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of hamster IL10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 101
    Range % 91-105
    1:2 Average % 106
    Range % 97-110
    1:4 Average % 95
    Range % 87-99
    1:8 Average % 93
    Range % 84-97
  • 回收率：
    The recovery of hamster IL10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 93 88-97
    EDTA plasma (n=4) 95 90-101
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    400 1.748 1.798 1.773 1.559
    200 1.302 1.343 1.323 1.109
    100 0.939 0.973 0.956 0.742
    50 0.710 0.699 0.705 0.491
    25 0.488 0.511 0.500 0.286
    12.5 0.409 0.417 0.413 0.199
    6.25 0.311 0.323 0.317 0.103
    0 0.212 0.216 0.214 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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