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  4. Hamster Interleukin 6,IL-6 ELISA Kit

Hamster Interleukin 6,IL-6 ELISA Kit

  • 中文名稱：
    倉鼠白介素6(IL-6)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E14304Ha
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    倉鼠白介素6(IL-6)酶聯(lián)免疫試劑盒（CSB-E14304Ha）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的IL-6含量。IL-6是重要細胞因子，在免疫調節(jié)、炎癥反應等生理病理過程中起關鍵作用。其過度表達與多種疾病如類風濕關節(jié)炎、新冠肺炎等密切相關。研究機制主要圍繞抑制IL-6及其受體信號通路，以調控炎癥反應和細胞增殖等，為疾病治療提供方向。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，科研人員可將其應用于炎癥機制研究、免疫治療模型評估或藥物研發(fā)中的細胞因子動態(tài)監(jiān)測，特別適合體外實驗環(huán)境下的定量分析需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    IL-6
  • 種屬：
    Mesocricetus auratus (Golden hamster)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    7.8 pg/mL-500 pg/mL
  • 靈敏度：
    1.95 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of hamster IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %87
    Range %82-92
    1:2Average %95
    Range %91-99
    1:4Average %96
    Range %91-100
    1:8Average %94
    Range %86-98
  • 回收率：
    The recovery of hamster IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9994-104
    EDTA plasma (n=4)9185-95
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    5002.576 2.456 2.516 2.351
    2502.107 2.076 2.092 1.927
    1251.423 1.417 1.420 1.255
    62.50.730 0.679 0.705 0.540
    31.20.457 0.470 0.464 0.299
    15.60.326 0.308 0.317 0.152
    7.80.262 0.247 0.255 0.090
    00.161 0.168 0.165 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價