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  4. Horse Fibrinogen(Fbg)ELISA Kit

Horse Fibrinogen(Fbg)ELISA Kit

  • 中文名稱：
    馬血纖蛋白原(Fbg)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E17079Hs
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    馬血纖蛋白原(Fbg)酶聯(lián)免疫試劑盒（CSB-E17079Hs）為競爭法ELISA試劑盒，定量檢測血清、血漿、尿液樣本中的Fbg含量。Fbg即纖維蛋白原，在凝血過程中發(fā)揮關(guān)鍵作用。其背景在于它是血漿中的一種重要蛋白質(zhì)。研究機制主要圍繞其在凝血級聯(lián)反應(yīng)里與凝血酶結(jié)合形成纖維蛋白，促進(jìn)血栓形成，在血栓性疾病研究及藥物研發(fā)中是重要靶點。試劑盒檢測范圍為23.4 ng/mL-1500 ng/mL，本試劑盒采用高特異性抗體，通過預(yù)包被板與樣本中Fbg競爭結(jié)合標(biāo)記物實現(xiàn)定量分析，操作流程標(biāo)準(zhǔn)化，適用于體外科研場景中凝血機制研究、疾病模型生物標(biāo)志物分析及藥效評估等實驗，例如通過檢測不同生理/病理狀態(tài)下體液樣本的Fbg水平變化，探究凝血-炎癥級聯(lián)反應(yīng)的分子機制或藥物干預(yù)效果。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    Fbg
  • 種屬：
    Equus caballus (Horse)
  • 樣本類型：
    serum, plasma, urine
  • 檢測范圍：
    23.4 ng/mL-1500 ng/mL
  • 靈敏度：
    23.4 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of horse fibrinogen in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:5000

    Average %

    93

    Range %

    88-99

    1:10000

    Average %

    97

    Range %

    91-105

    1:20000

    Average %

    89

    Range %

    82-96

    1:40000

    Average %

    96

    Range %

    90-102

  • 回收率：

    The recovery of horse fibrinogen spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    95

    89-98

    EDTA plasma (n=4)

    98

    93-103

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    1500

    0.149

    0.154

    0.151

    750

    0.226

    0.237

    0.231

    375

    0.301

    0.320

    0.310

    187.5

    0.436

    0.429

    0.432

    93.75

    0.597

    0.611

    0.604

    46.8

    0.876

    0.853

    0.865

    23.4

    1.294

    1.284

    1.289

    0

    2.338

    2.225

    2.282

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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