Mouse Fibrinogen,Fbg ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (5) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠血纖蛋白原(Fbg)酶聯(lián)免疫試劑盒（CSB-E08202m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Fbg含量。Fbg即纖維蛋白原，在凝血過程中起關(guān)鍵作用。其與血栓形成緊密相關(guān)，過高水平易致血栓性疾病。研究機(jī)制主要圍繞其在凝血級聯(lián)反應(yīng)中的作用、與血小板相互作用等方面，探索調(diào)節(jié)其功能可開發(fā)抗血栓等相關(guān)治療策略。試劑盒檢測范圍為0.156 μg/mL-10 μg/mL，適用于小鼠凝血機(jī)制研究、血栓性疾病模型評估、肝臟功能分析或創(chuàng)傷修復(fù)相關(guān)實(shí)驗(yàn)，可為心血管疾病、出血性疾病及炎癥反應(yīng)等科研領(lǐng)域提供可靠的檢測工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

Fbg
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.156 μg/mL-10 μg/mL
靈敏度：

0.039 μg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Fbg in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	95
	Range %	87-103
1:400	Average %	92
	Range %	84-105
1:800	Average %	96
	Range %	83-106
1:1600	Average %	93
	Range %	88-104

回收率：

The recovery of mouse Fbg spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-108
EDTA plasma (n=4)	96	88-107

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.