Horse platelet-derived growth factor-BB(PDGF-BB) ELISA kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

馬血小板衍生生長(zhǎng)因子BB(PDGF-BB)酶聯(lián)免疫試劑盒（CSB-EQ027241HO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿,組織勻漿樣本中的PDGF-BB含量。PDGF - BB即血小板衍生生長(zhǎng)因子 - BB，在細(xì)胞生長(zhǎng)、增殖、遷移及血管生成等生理過程中發(fā)揮重要作用。其異常表達(dá)與腫瘤、心血管疾病等多種病理狀況相關(guān)。研究聚焦于其信號(hào)通路機(jī)制，期望通過干預(yù)該靶點(diǎn)開發(fā)新的治療藥物和策略。試劑盒檢測(cè)范圍為1.56 ng/mL-100 ng/mL，適用于科研場(chǎng)景中探究心血管疾病模型、創(chuàng)傷修復(fù)機(jī)制或干細(xì)胞分化調(diào)控等相關(guān)課題，為體外實(shí)驗(yàn)中PDGF-BB的功能研究提供可靠的數(shù)據(jù)支持本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

PDGF-BB
種屬：

Equus caballus (Horse)
樣本類型：

serum, plasma,tissue homogenates
檢測(cè)范圍：

1.56 ng/mL-100 ng/mL
靈敏度：

0.39 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cardiovascular
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of horse PDGF-BB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-91
1:2	Average %	94
	Range %	91-99
1:4	Average %	84
	Range %	81-87
1:8	Average %	83
	Range %	80-86

回收率：

The recovery of horse PDGF-BB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	101-109
EDTA plasma (n=4)	94	91-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.