Mouse platelet-derived growth factor-BB,PDGF-BB ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (6) 說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠血小板衍生生長(zhǎng)因子BB(PDGF-BB)酶聯(lián)免疫試劑盒（CSB-E08925m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的PDGF-BB含量。PDGF-BB 是血小板衍生生長(zhǎng)因子異構(gòu)體。其背景在于它在細(xì)胞生長(zhǎng)、增殖等生理過(guò)程有關(guān)鍵作用。研究機(jī)制主要圍繞其與受體結(jié)合，激活下游信號(hào)通路，在腫瘤血管生成、纖維化疾病發(fā)展等病理過(guò)程有重要影響，也是藥物研發(fā)熱門(mén)靶點(diǎn)。試劑盒檢測(cè)范圍為0.18 ng/mL-12 ng/mL，科研人員可將其應(yīng)用于小鼠模型中的炎癥反應(yīng)研究、血管生成機(jī)制探索或組織再生相關(guān)實(shí)驗(yàn)，也可用于評(píng)估藥物干預(yù)或基因修飾對(duì) PDGF-BB 表達(dá)的影響。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

PDGF-BB
種屬：

Mus musculus (Mouse)
樣本類(lèi)型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

0.18 ng/mL-12 ng/mL
靈敏度：

0.047 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cardiovascular
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PDGF-BB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	82-88
1:2	Average %	94
	Range %	89-98
1:4	Average %	104
	Range %	97-109
1:8	Average %	99
	Range %	93-105

回收率：

The recovery of mouse PDGF-BB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-101
EDTA plasma (n=4)	89	82-96

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.