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  4. Human β-hexosaminidase A,β-Hex A ELISA Kit

Human β-hexosaminidase A,β-Hex A ELISA Kit

  • 中文名稱：
    人β氨基己糖苷酶A(β-Hex A)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E09462h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人β氨基己糖苷酶A(β-Hex A)酶聯(lián)免疫試劑盒（CSB-E09462h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的βHex A含量。βHex A是一種重要靶點(diǎn)。它在某些疾病發(fā)病機(jī)制中起關(guān)鍵作用，如泰-薩克斯病等。研究機(jī)制方面，主要聚焦于其在細(xì)胞內(nèi)代謝產(chǎn)物的清除過程，通過解析其結(jié)構(gòu)與功能，試圖尋找調(diào)節(jié)它的方法，以研發(fā)治療相關(guān)疾病的藥物。試劑盒檢測(cè)范圍為1.56 mIU/mL-100 mIU/mL，該產(chǎn)品適用于科研領(lǐng)域中對(duì)β - Hex A表達(dá)水平的定量分析，可用于細(xì)胞模型、動(dòng)物實(shí)驗(yàn)或人體生物樣本中酶活性變化的研究，為探索溶酶體貯積癥發(fā)病機(jī)制、代謝通路調(diào)控或潛在治療策略的體外評(píng)估提供高效工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    beta-hexosaminidase A, beta-Hex A
  • 縮寫：
    βHex A
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma
  • 檢測(cè)范圍：
    1.56 mIU/mL-100 mIU/mL
  • 靈敏度：
    0.39 mIU/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human β-Hex A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 90  
    Range % 85-94  
    1:2 Average % 93  
    Range % 88-94  
    1:4 Average % 105  
    Range % 100-112  
    1:8 Average % 86  
    Range % 80-92  
  • 回收率：
    The recovery of human β-Hex A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 93-102  
    EDTA plasma (n=4) 92 88-96  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/ml OD1 OD2 Average Corrected  
    100 2.838 2.972 2.905 2.782  
    50 2.140 2.175 2.158 2.035  
    25 1.416 1.406 1.411 1.288  
    12.5 0.903 0.895 0.899 0.776  
    6.25 0.516 0.502 0.509 0.386  
    3.12 0.330 0.353 0.342 0.219  
    1.56 0.264 0.281 0.273 0.150  
    0 0.119 0.127 0.123    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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