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  4. Human β2-microglobulin,BMG/β2-MG ELISA Kit

Human β2-microglobulin,BMG/β2-MG ELISA Kit

  • 中文名稱：
    人β2微球蛋白(BMG/β2-MG)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E04883h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人β2微球蛋白(BMG/β2-MG)酶聯(lián)免疫試劑盒（CSB-E04883h）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、尿液樣本中的B2M含量。B2M即β2-微球蛋白，它是MHCⅠ類分子的輕鏈，廣泛存在于有核細(xì)胞表面。在腫瘤免疫等研究機(jī)制中，B2M參與抗原呈遞過程。其表達(dá)異常與多種疾病相關(guān)，如腫瘤免疫逃逸等，深入研究有助于理解疾病發(fā)病機(jī)制并為治療提供新靶點(diǎn)。試劑盒檢測(cè)范圍為0.039 μg/mL-10 μg/mL，靈敏度為0.039 μg/mL。適用于科研場(chǎng)景中對(duì)β2 - MG的動(dòng)態(tài)監(jiān)測(cè)，如探索腎臟疾病進(jìn)展機(jī)制、評(píng)估腫瘤微環(huán)境中免疫調(diào)節(jié)作用，或研究自身免疫性疾病中細(xì)胞代謝異常等方向本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    B2M ELISA Kit; B2MG_HUMAN ELISA Kit; Beta 2 microglobin ELISA Kit; Beta 2 microglobulin ELISA Kit; Beta 2 microglobulin precursor ELISA Kit; Beta chain of mhc class 1 proteins ELISA Kit; Beta chain of MHC class I molecules ELISA Kit; Beta-2-microglobulin form pI 5.3 ELISA Kit; CDABP0092 ELISA Kit; Hdcma22p ELISA Kit; IMD43 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, urine
  • 檢測(cè)范圍：
    0.039 μg/mL-10 μg/mL
  • 靈敏度：
    0.039 μg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human β2-MG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    86

    Range %

    81-92

    1:2

    Average %

    85

    Range %

    80-92

    1:4

    Average %

    87

    Range %

    81-96

    1:8

    Average %

    89

    Range %

    84-95

  • 回收率：

    The recovery of human β2-MG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    91

    87-95

    EDTA plasma (n=4)

    87

    83-96

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    μg/ml

    OD1

    OD2

    Average

    10

    0.315

    0.310

    0.312

    2.5

    0.716

    0.722

    0.719

    0.625

    1.174

    1.128

    1.151

    0.156

    1.648

    1.629

    1.638

    0.039

    1.938

    1.821

    1.880

    0

    2.066

    2.183

    2.125

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 最新研究進(jìn)展：
        B2M（β2微球蛋白）是MHC-I分子的組成部分，廣泛表達(dá)于多種細(xì)胞類型中，包括淋巴細(xì)胞、肝細(xì)胞和腎小球。B2M在腫瘤、自身免疫和感染等疾病中發(fā)揮重要作用，可以作為某些癌癥和自身免疫性疾病的潛在治療靶點(diǎn)。
     
  • 功能：
    Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation.
  • 基因功能參考文獻(xiàn)：
    1. Data show that plasma beta2-microglobulin (beta2m) levels are specifically increased in Alzheimer's disease (AD), indicating that beta2m could play a role in AD. PMID: 29741401
    2. Binding stability to beta2-microglobulin may confer to HLA-C the ability to preferentially act either as a conventional immune-competent molecule or as an accessory molecule involved in HIV-1 infectivity via viral envelope glycoprotein binding. PMID: 28051183
    3. Molecular dynamic simulations reveal that D59P mutation in the DE loop region of B2M disrupts the native sandwich folded structure and highlight a decrease in the average number of hydrogen bonds in the loop regions on D59P mutation that enhances conformational flexibility, which lead to higher aggregation propensity. PMID: 28657666
    4. Study characterized the salt-induced oligomers of the acid-unfolded state of beta2m using an array of biophysical tools including steady-state and time-resolved fluorescence, circular dichroism, dynamic light scattering, and atomic force microscopy imaging. Results revealed the distinct pathways by which beta2m assembles into oligomers and fibrils. PMID: 27467899
    5. We focused on the genetic and functional analysis of beta2-microglobulin (B2M), a component of the HLA class-I complex. We identified alterations in genes involved in various functions, such as B2M involved in immunosurveillance PMID: 28302866
    6. Pretransplant beta2-Microglobulin Is Associated with the Risk of Acute Graft-versus-Host-Disease after Allogeneic Hematopoietic Cell Transplant.( PMID: 27044906
    7. we found that beta2M but no other serum markers had independent prognostic significance in the UK CLL4 trial PMID: 26732125
    8. Both cystatin C and beta 2 macroglobulin are elevated in type 2 diabetics with diabetic nephropathy. PMID: 28182344
    9. Serum B2M level was positively associated with Gensini score system, SYNTAX score system and the number of diseased coronary vessels. PMID: 28249620
    10. Data suggest that T-cell receptor (TCR) alpha-constant domain (C- and F-strands) directly interacts with unbound MHC light chain, beta2-microglobulin (beta2M); these interactions do not appear to be involved in T-cell cytotoxicity, suggesting an alternate role for beta2M binding to TCR; TCR beta-chain does not appear to interact directly with beta2M. PMID: 28671821
    11. These data suggest beta2M might participate in the development of lung emphysema through induction of lung epithelial cell senescence and inhibition. PMID: 28213472
    12. Heparin-induced amyloid fibrillation of beta2 -microglobulin explained by solubility and a supersaturation-dependent conformational phase diagram PMID: 28249361
    13. results suggest that doxycycline and rifamycin are general inhibitors of Cu(II)-induced beta2m amyloid formation. PMID: 28468825
    14. High B2M levels were associated with an increased risk of ischemic stroke in this study of middle-aged women PMID: 28490653
    15. Letter: serum levels of anti-carbonic anhydrase autoantibodies and beta macroglobulin correlate with ClinESSDAI score in patients with Sjogren's syndrome. PMID: 28079504
    16. Macromolecular crowding favors the fibrillization of beta2-microglobulin by accelerating the nucleation step and inhibiting fibril disassembly. PMID: 27481166
    17. Beta2M mRNA expression in cells of the urinary sediment is higher in T1D patients with diabetic kidney disease and in patients with focal segmental glomerulosclerosis in comparison to healthy subjects, maybe reflecting a tubulointerstitial injury promoted by albumin. PMID: 27173460
    18. Serum levels of beta trace protein and beta 2 microglobulin can be used to predict residual kidney function in hemodialysis patients. PMID: 26924065
    19. Results show that high serum 2-m levels and lymphoma associated hemophagocytic lymphohistiocytosis (LAHS) were associated with markedly poorer overall survival (OS) in patients with hemophagocytic lymphohistiocytosis suggesting that serum2-m concentration was a powerful and independent prognostic factor for OS in patients with LAHS. PMID: 27110054
    20. The chaperone crystallin, which is a mild modulator of the lag phase of the variant fibrillogenesis, potently inhibits fibril elongation of the wild-type even once it is absorbed on D76N beta2-microglobulin fibrils. PMID: 26921323
    21. B2M is a potential tissue biomarker and therapeutic target of borderline and malignant ovarian tumours and the dysregulation of B2M in these tumours may be mediated by the TGF-beta signaling pathway. PMID: 26983758
    22. BTP and B2M levels are less affected than serum by amputation, and should be considered for future study of GFR estimation PMID: 26800100
    23. Overexpression of B2M are associated with invasion, metastasis of the pancreatic ductal adenocarcinoma. PMID: 26406402
    24. Serum Beta-2 Microglobulin level is elevated in Chronic Hepatitis C related chronic liver diseases and may be used as a marker for Chronic Hepatitis C disease progression towards cirrhosis and carcinoma. PMID: 26625805
    25. Data suggest that, in modeling early assembly mechanisms for amyloidogenic beta2-microglobulin (B2M and/or truncated B2M, crosslinked and native), domain-swapped hinge region of B2M is key to both intra-molecular and inter-molecular interactions. PMID: 26655720
    26. The results show that, although other factors influence the fibril growth kinetics, a striking difference in the solubility of the proteins is a key determinant of the different amyloidogenicity of human B2M and mouse B2M. PMID: 26780548
    27. beta2M-mediated VEGFR-2/Akt/mTOR phosphorylation and tumor angiogenesis was significantly suppressed by over-expression of DKK-3. PMID: 26278164
    28. ESAT-6/ESAT-6:CFP-10 can enter into the endoplasmic reticulum where it sequesters beta2M to inhibit cell surface expression of MHC-I-beta2M complexes, resulting in downregulation of class I-mediated antigen presentation. PMID: 25356553
    29. The study presents the X-ray crystallographic structures of three families of oligomers formed by macrocyclic peptides containing a heptapeptide sequence derived from the amyloidogenic E chain of beta 2-microglobulin (B2M). PMID: 25915729
    30. Serum cystatin C, urine NAG, RBP, and ET-1 levels were found to be insufficient for the evaluation of SCD nephropathy. Increased B2M/creatinie levels can be valuable in estimating possible glomerular and tubular damage in SCD. PMID: 23987825
    31. Unfolding of the C-terminal portion of human beta 2 microglobulin may be related to amyloidogenicity. PMID: 25523495
    32. The results suggest that the interaction between beta2m fibrils and membranes of endosomal origin may play a role in the molecular mechanism of beta2m amyloid-associated osteoarticular tissue destruction in DRA. PMID: 25100247
    33. Monoclonal antibodies to HLA-E bind epitopes carried by unfolded beta2 m-free heavy chains. PMID: 25982269
    34. Beta2-Microglobulin deficiency causes a complex immunodeficiency of the innate and adaptive immune system. PMID: 25702838
    35. A competitive mechanism of amyloid fibrillation and amorphous aggregation reproduced the observed aggregation kinetics of beta2-microglobulin. PMID: 26063798
    36. Conformational differences between B2M and its truncated variant DeltaN6 fibrils can explain the relatively enhanced amyloidogenic potential of the truncation variant. PMID: 24679070
    37. findings suggest that beta2m at transplantation predict the outcome after ASCT in NHL PMID: 25577449
    38. The expression profiles of DEP1 and B2MG correlate with increased cell senescence and survival in breast cancer. PMID: 25412306
    39. Beta 2-microglobulin is responsible for systemic amyloidosis in patients undergoing long-term hemodialysis. PMID: 25750126
    40. beta2-M expression demonstrated a significant difference in the four breast cancer molecular subtypes, and may be related to apoptosis regulation in breast cancer. PMID: 25292288
    41. Serum B2-M levels may be used as an activity parameter in inflammatory bowel disease. PMID: 25152594
    42. Urinary beta2-MG levels showed a significant correlation with renal function and proteinuria in IgA nephropathy. PMID: 24851068
    43. results suggest that serum beta2M and GGT levels are useful indicators for evaluating the occurrence of slight inflammation as background, which promotes atherosclerosis even in healthy children. PMID: 24572983
    44. Beta-2 microglobulin levels are increased in about 30 percent of renal cell carcinoma patients, above all in subjects with metastatic disease. PMID: 24692843
    45. Substantial changes of CSF beta 2-m concentrations are predictive of purulent meningitis, leptomeningeal metastasis, viral meningitis/encephalitis, and neuroborreliosis. PMID: 24895473
    46. Serum beta2-microglobulin levels correlate with tumor stage in MM. PMID: 25247952
    47. The increased proportion of advanced glycation end products and beta2M deposition induced by long-term exposure of peritoneal dialysis fluid may be a marker of peritoneal injury. PMID: 24337623
    48. Amyloidogenic and nonamyloidogenic variants of beta2-microglobulin, mapping interactions that inhibit or promote fibril formation in atomic detail. PMID: 24981172
    49. MHC I and b2m genes are both expressed in in human as well as mice during the developmental stages of cerebellar cortex PMID: 24272393
    50. An association between serum B2M and non-Hodgkin lymphoma prognosis. PMID: 24969158

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  • 相關(guān)疾?。?/div>
    Immunodeficiency 43 (IMD43); Amyloidosis 8 (AMYL8)
  • 亞細(xì)胞定位：
    Secreted. Cell surface.;
  • 蛋白家族：
    Beta-2-microglobulin family
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 914

    OMIM: 105200

    KEGG: hsa:567

    STRING: 9606.ENSP00000452780

    UniGene: Hs.534255