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  4. Human 4-Hydroxynonenal (HNE)ELISA Kit

Human 4-Hydroxynonenal (HNE)ELISA Kit

  • 中文名稱：
    人4-羥基壬烯酸(HNE)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E16214h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人4-羥基壬烯酸(HNE)酶聯(lián)免疫試劑盒（CSB-E16214h）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的HNE含量。人中性粒細胞彈性蛋白酶（HNE）是一種絲氨酸蛋白酶，存在于中性粒細胞嗜天青顆粒中。HNE與多種疾病如肺氣腫、囊性纖維化等密切相關(guān)。研究機制主要圍繞抑制其活性來開發(fā)藥物，以減輕因HNE過度表達引發(fā)的炎癥和組織損傷。試劑盒檢測范圍為39 pg/mL-2500 pg/mL，適用于基礎(chǔ)科研中氧化應(yīng)激相關(guān)疾病模型的機制研究、藥物或天然產(chǎn)物的抗氧化功效評價，以及代謝組學(xué)中脂質(zhì)過氧化程度的動態(tài)監(jiān)測，為探索HNE在生理病理過程中的調(diào)控作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    HNE
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    39 pg/mL-2500 pg/mL
  • 靈敏度：
    19.5 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human HNE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:100

    Average %

    89

    Range %

    82-96

    1:200

    Average %

    90

    Range %

    85-96

    1:400

    Average %

    85

    Range %

    81-92

    1:800

    Average %

    98

    Range %

    91-104

  • 回收率：

    The recovery of human HNE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    94

    87-101

    EDTA plasma (n=4)

    98

    91-104

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    2500

    0.175

    0.170

    0.173

    1250

    0.261

    0.273

    0.267

    625

    0.383

    0.408

    0.396

    312.5

    0.669

    0.629

    0.649

    156.25

    1.008

    1.021

    1.014

    78

    1.345

    1.353

    1.349

    39

    1.879

    1.784

    1.832

    0

    2.611

    2.525

    2.568

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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