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Human 8-Hydroxydeoxyguanosine (8-OHdG) ELISA kit

  • 中文名稱(chēng):
    人8羥基脫氧鳥(niǎo)苷(8-OHdG)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E10140h
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人8羥基脫氧鳥(niǎo)苷(8-OHdG)酶聯(lián)免疫試劑盒(CSB-E10140h)為競(jìng)爭(zhēng)法ELISA試劑盒,定量檢測(cè)血清、尿液樣本中的8-OHdG含量。8-OHdG是細(xì)胞內(nèi)DNA氧化應(yīng)激損傷的標(biāo)志性產(chǎn)物。其背景在于機(jī)體受內(nèi)外部因素影響產(chǎn)生氧化應(yīng)激,損傷DNA生成8-OHdG。研究機(jī)制方面,常檢測(cè)其水平以評(píng)估氧化應(yīng)激程度,在疾病早期診斷、病情監(jiān)測(cè)及藥物研發(fā)等領(lǐng)域有重要意義。試劑盒檢測(cè)范圍為 3.12 ng/mL-800 ng/mL,該產(chǎn)品適用于體外定量評(píng)估生物樣本中的氧化損傷水平,為探索氧化應(yīng)激與疾病關(guān)聯(lián)機(jī)制、藥物篩選及毒理學(xué)研究提供高效工具,尤其適合需要高靈敏度和寬線(xiàn)性范圍的科研場(chǎng)景,滿(mǎn)足血清和尿液等易獲取樣本的便捷檢測(cè)需求。在衰老、腫瘤發(fā)生、神經(jīng)退行性疾病及環(huán)境毒理等研究領(lǐng)域具有重要科研價(jià)值。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě):
    8-OHdG
  • 種屬:
    Homo sapiens (Human)
  • 樣本類(lèi)型:
    serum, urine
  • 檢測(cè)范圍:
    3.12 ng/mL-800 ng/mL
  • 靈敏度:
    3.12 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Cell Biology
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Competitive
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<12%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<15%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線(xiàn)性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human 8-OHdG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:1

    Average %

    84

    Range %

    80-91

    1:2

    Average %

    87

    Range %

    81-95

    1:4

    Average %

    88

    Range %

    82-95

    1:8

    Average %

    86

    Range %

    81-92

  • 回收率:

    The recovery of human 8-OHdG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    89

    84-96

  • 標(biāo)準(zhǔn)曲線(xiàn):

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    800

    0.337

    0.344

    0.340

    0.340

    200

    0.873

    0.857

    0.865

    0.865

    50

    1.302

    1.328

    1.315

    1.315

    12.5

    1.784

    1.699

    1.741

    1.741

    3.12

    2.190

    2.291

    2.240

    2.240

    0

    2.534

    2.453

    2.493

    2.493

  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 最新研究進(jìn)展:
        8-OHdG是DNA氧化損傷的標(biāo)志物,最新研究表明,其與各種疾病的發(fā)生和發(fā)展有關(guān),包括癌癥、糖尿病、阿爾茨海默病等。此外,8-OHdG的水平也可以用作生活方式和環(huán)境暴露的生物標(biāo)志物。