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  4. Mouse 8-Hydroxy-desoxyguanosine,8-OHdG ELISA Kit

Mouse 8-Hydroxy-desoxyguanosine,8-OHdG ELISA Kit

  • 中文名稱：
    小鼠8羥基脫氧鳥(niǎo)苷(8-OHdG)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E10527m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠8羥基脫氧鳥(niǎo)苷(8-OHdG)酶聯(lián)免疫試劑盒（CSB-E10527m）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的8-OHdG含量。8-OHdG 是 DNA 氧化損傷的生物標(biāo)志物。其背景在于機(jī)體受內(nèi)外界因素影響產(chǎn)生氧化應(yīng)激，導(dǎo)致 DNA 中鳥(niǎo)嘌呤氧化形成 8-OHdG。研究機(jī)制多圍繞檢測(cè)其含量來(lái)評(píng)估氧化應(yīng)激水平、DNA 損傷程度，及與腫瘤、神經(jīng)退行性疾病等的關(guān)聯(lián)。試劑盒檢測(cè)范圍為0.78 pg/mL-50 pg/mL，適用于神經(jīng)退行性疾病模型、衰老機(jī)制研究、環(huán)境毒理學(xué)評(píng)估及代謝性疾病等科研領(lǐng)域，可為氧化損傷相關(guān)機(jī)制研究、藥物篩選及抗氧化劑功效評(píng)價(jià)提供數(shù)據(jù)支持，廣泛應(yīng)用于基礎(chǔ)醫(yī)學(xué)、藥理學(xué)及生物化學(xué)等科研場(chǎng)景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě)：
    8-OHdG
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.78 pg/mL-50 pg/mL
  • 靈敏度：
    0.195 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cell Biology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse 8-OHdG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 90  
    Range % 87-97  
    1:200 Average % 97  
    Range % 93-104  
    1:400 Average % 85  
    Range % 82-91  
    1:800 Average % 90  
    Range % 85-97  
  • 回收率：
    The recovery of mouse 8-OHdG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 89 85-93  
    EDTA plasma (n=4) 92 88-94  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    50 0.071 0.074 0.073    
    25 0.120 0.130 0.125    
    12.5 0.200 0.206 0.203    
    6.25 0.335 0.340 0.338    
    3.12 0.573 0.554 0.564    
    1.56 0.858 0.841 0.850    
    0.78 1.129 1.139 1.134    
    0 2.215 2.185 2.200    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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