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  4. Human Acid sphingomyelinase-like phosphodiesterase 3b(SMPDL3B) ELISA kit

Human Acid sphingomyelinase-like phosphodiesterase 3b(SMPDL3B) ELISA kit

  • 中文名稱：
    人酸性鞘磷脂樣磷酸二酯酶3b(SMPDL3B)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL021850HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人酸性鞘磷脂樣磷酸二酯酶3b(SMPDL3B)酶聯(lián)免疫試劑盒（CSB-EL021850HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的SMPDL3B含量。SMPDL3B是一種脂質(zhì)調(diào)節(jié)磷酸二酯酶，主要在巨噬細(xì)胞和樹突狀細(xì)胞表面活性，影響巨噬細(xì)胞脂質(zhì)組成和膜流動性。其通過破壞鞘脂膜代謝，促進(jìn)脂肪性肝炎，并作為Toll樣受體信號通路的負(fù)調(diào)節(jié)因子，在免疫應(yīng)答中發(fā)揮作用。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，該產(chǎn)品可為科研人員開展脂質(zhì)代謝機制、腎臟病理研究、免疫調(diào)節(jié)相關(guān)信號通路探索提供可靠工具，尤其適用于體外模型中SMPDL3B蛋白表達(dá)量變化的定量分析，為疾病機理研究和藥物開發(fā)建立基礎(chǔ)實驗數(shù)據(jù)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    SMPDL3B ELISA kit; ASML3B ELISA kit; ASMLPDAcid sphingomyelinase-like phosphodiesterase 3b ELISA kit; ASM-like phosphodiesterase 3b ELISA kit; EC 3.1.4.- ELISA kit
  • 縮寫：
    SMPDL3B
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    0.625 ng/mL-40 ng/mL
  • 靈敏度：
    0.156 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human SMPDL3B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 86
    Range % 83-89
    1:2 Average % 92
    Range % 89-97
    1:4 Average % 100
    Range % 95-105
    1:8 Average % 90
    Range % 87-93
  • 回收率：
    The recovery of human SMPDL3B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 104 100-108
    EDTA plasma (n=4) 99 95-103
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    40 2.243 2.102 2.173 2.031
    20 1.545 1.632 1.589 1.447
    10 1.149 1.201 1.175 1.033
    5 0.825 0.835 0.830 0.688
    2.5 0.505 0.531 0.518 0.376
    1.25 0.345 0.362 0.354 0.212
    0.625 0.224 0.215 0.220 0.078
    0 0.144 0.140 0.142 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Lipid-modulating phosphodiesterase. Active on the surface of macrophages and dendritic cells and strongly influences macrophage lipid composition and membrane fluidity. Acts as a negative regulator of Toll-like receptor signaling. Has in vitro phosphodiesterase activity, but the physiological substrate is unknown. Lacks activity with phosphocholine-containing lipids, but can cleave CDP-choline, and can release phosphate from ATP and ADP (in vitro).
  • 基因功能參考文獻(xiàn)：
    1. Glomerular immunoreactivity and urinary excretion of SMPDL3b in proteinuric nephrotic syndrome patients decreased compared with controls. PMID: 29081073
    2. These results demonstrate that radiation injury induces early cytoskeletal remodeling, down-regulation of SMPDL3b, and elevation of cellular ceramide levels. Overexpression of SMPDL3b and pretreatment with rituximab confer a radioprotective effect in cultured podocytes. PMID: 27836988
  • 亞細(xì)胞定位：
    Secreted. Cell membrane; Lipid-anchor, GPI-anchor.
  • 蛋白家族：
    Acid sphingomyelinase family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 21416

    OMIM: 617737

    KEGG: hsa:27293

    STRING: 9606.ENSP00000363001

    UniGene: Hs.123659