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  4. Human Adipsin ELISA Kit

Human Adipsin ELISA Kit

  • 中文名稱：
    人降脂素酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E14369h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人降脂素酶聯(lián)免疫試劑盒（CSB-E14369h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CFD含量。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，靈敏度為0.078 ng/mL。適用于基礎(chǔ)醫(yī)學(xué)研究中脂代謝調(diào)控機(jī)制探索、心血管疾病模型分析、以及降脂藥物或營養(yǎng)干預(yù)的體外效果評估；支持科研人員高效完成細(xì)胞實驗、動物模型或臨床前樣本的定量分析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Adipsin ELISA Kit; Adipsin/complement factor D ELISA Kit; Adn ELISA Kit; C3 convertase activator ELISA Kit; CFAD_HUMAN ELISA Kit; CFD ELISA Kit; Complement factor D ELISA Kit; complement factor D preproprotein ELISA Kit; D component of complement ELISA Kit; DF ELISA Kit; FactorD ELISA Kit; PFD ELISA Kit; Properdin factor D ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

    　

    Intra-Assay Precision

    Inter-Assay Precision

    Sample

    1

    2

    3

    1

    2

    3

    n

    20

    20

    20

    20

    20

    20

    Mean(ng/ml)

    2.593

    2.559

    2.619

    2.671

    2.640

    2.602

    SD

    0.043

    0.036

    0.039

    0.057

    0.050

    0.048

    CV(%)

    5.089

    4.301

    4.583

    6.605

    5.841

    5.667

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human adipsin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:500

    Average %

    87

    Range %

    82-91

    1:1000

    Average %

    84

    Range %

    80-88

    1:2000

    Average %

    104

    Range %

    98-108

    1:4000

    Average %

    90

    Range %

    85-94

  • 回收率：

    The recovery of human adipsin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    98

    94-103

    EDTA plasma (n=4)

    94

    90-100

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    20

    2.591

    2.423

    2.507

    2.402

    10

    1.964

    1.890

    1.927

    1.822

    5

    1.336

    1.301

    1.319

    1.214

    2.5

    0.842

    0.836

    0.839

    0.734

    1.25

    0.449

    0.424

    0.437

    0.332

    0.63

    0.329

    0.339

    0.334

    0.229

    0.31

    0.249

    0.241

    0.245

    0.140

    0

    0.108

    0.102

    0.105

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 最新研究進(jìn)展：
    CFD（Complement factor D）是補(bǔ)體系統(tǒng)中的一種血清蛋白，主要參與剪切促進(jìn)因子B（Factor B）的激活，從而啟動替代途徑的活化。最新研究表明，CFD在炎癥、自身免疫疾病、感染和腫瘤等多種疾病中都起到了重要作用。例如，CFD可以促進(jìn)炎癥細(xì)胞的浸潤和激活，導(dǎo)致組織損傷和炎癥反應(yīng)。此外，CFD也參與了腫瘤的生長、侵襲和轉(zhuǎn)移等過程。
     
  • 功能：
    Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.
  • 基因功能參考文獻(xiàn)：
    1. MASP-3 is the exclusive pro-factor D activator in resting blood: the lectin and the alternative complement pathways are fundamentally linked. PMID: 27535802
    2. disturbance of reciprocal relationships between adipsin and leptin in obesity is associated with the development of insulin resistance PMID: 28864895
    3. TZDs (pioglitazone, rosiglitazone, and ciglitazone) slow tumor cell growth in vitro and in vivo with decreases in cell proliferation and increases in PPARg and adipsin PMID: 28219679
    4. The strong association of PLIN1, CFD and ADIPOQ genes with adipogenesis prompted authors to study the influence the bone health status as evaluated by quantitative ultrasound (QUS) bone densitometer in a North Indian cohort. Overall, ADIPOQ (rs1501299 and rs3774261) and combined cluster of PLIN1 rs2304796 and rs2304795) and CFD (rs1683563) demonstrated correlation. PMID: 28189761
    5. our findings set up a novel mechanism for FABP4, adipsin and adiponectin through gut microbiota mediating expression in gut Paneth cells. PMID: 26687459
    6. Overall adipsin levels among male asbestos industry workers were significantly higher than among control subjects. PMID: 25840635
    7. Data indicate that a urinary protein, adipsin, was significantly increased in patients with preeclampsia. PMID: 24958499
    8. Study demonstrates that T2DM patients with beta cell failure are deficient in adipsin. PMID: 24995977
    9. Complement factor D (FD) is activated by its substrate through interactions outside the active site which lock the unbound native state into an ordered inactive conformation via the self-inhibitory loop in FD. PMID: 24598742
    10. We demonstrate novel secretion of adipsin and ASP by placental Hofbauer cells. PMID: 23956345
    11. Increased activation of the alternative complement pathway in vitreous was controlled by disease stage and genetic variation in the complement pathway, supporting a role for complement activation in macular degeneration disease pathogenesis. PMID: 22930722
    12. Anti factor D Fab fragment inhibits FD proteolytic function by interfering with macromolecular substrate access rather than by inhibiting FD catalysis. PMID: 22362762
    13. CFD regulates activation of the alternative complement pathway, which is implicated in age related macular degeneration pathogenesis. PMID: 22003108
    14. CFD may not play a major role in the genetic susceptibility to age-related macular degeneration. PMID: 21139680
    15. crystal structure of C3bB at 4 A and complex with factor D at 3.5 A; data show how factor B binding to C3b forms open "activation" state of C3bB; Factor D binds open conformation of factor B through a site distant from the catalytic center PMID: 21205667
    16. As adiponectin and adipsin levels in CSF did not correlate with their levels in plasma, it seems that there could be a secondary intrathecal synthesis of these adipocytokines in multiple sclerosis PMID: 19538214
    17. increased serum levels in patients with seasonal allergic rhinitis PMID: 19699327

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  • 相關(guān)疾?。?/div>
    Complement factor D deficiency (CFDD)
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase S1 family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 2771

    OMIM: 134350

    KEGG: hsa:1675

    STRING: 9606.ENSP00000332139

    UniGene: Hs.155597