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  4. Human Centromere protein K(CENPK) ELISA kit

Human Centromere protein K(CENPK) ELISA kit

  • 中文名稱：
    人著絲粒蛋白K(CENPK)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL005214HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人著絲粒蛋白K(CENPK)酶聯(lián)免疫試劑盒（CSB-EL005214HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的CENPK含量。CENPK（著絲粒蛋白K）是一種與著絲粒復(fù)合體相關(guān)的亞基，其功能是將CENPA蛋白靶向到著絲粒，對于著絲粒功能和有絲分裂進程至關(guān)重要。研究顯示，CENPK基因在肝細(xì)胞癌中異常高表達(dá)，其啟動子區(qū)域的DNA低甲基化水平與其高表達(dá)密切相關(guān)，提示表觀遺傳學(xué)機制在肝細(xì)胞癌發(fā)生發(fā)展中起重要作用。試劑盒檢測范圍為23.5 pg/mL-1500 pg/mL，可在科研場景中用于探索CENPK在細(xì)胞增殖、分化及癌癥發(fā)生發(fā)展中的分子機制，尤其適用于染色體動力學(xué)研究、腫瘤模型構(gòu)建以及藥物干預(yù)對染色體穩(wěn)定性影響的體外實驗，適用于分子生物學(xué)、腫瘤學(xué)等領(lǐng)域的蛋白功能研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    CENPK ELISA Kit; ICEN37 ELISA Kit; FKSG14 ELISA Kit; Centromere protein K ELISA Kit; CENP-K ELISA Kit; Interphase centromere complex protein 37 ELISA Kit; Protein AF-5alpha ELISA Kit; p33 ELISA Kit
  • 縮寫：
    CENPK
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    23.5 pg/mL-1500 pg/mL
  • 靈敏度：
    5.8 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CENPK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %93
    Range %88-97
    1:2Average %100
    Range %90-105
    1:4Average %92
    Range %85-96
    1:8Average %92
    Range %84-96
  • 回收率：
    The recovery of human CENPK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9185-95
    EDTA plasma (n=4)9990-102
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    15001.889 1.966 1.928 1.798
    7501.426 1.476 1.451 1.321
    3750.921 0.900 0.911 0.781
    187.50.566 0.543 0.555 0.425
    940.369 0.345 0.357 0.227
    470.259 0.263 0.261 0.131
    23.50.199 0.192 0.196 0.066
    00.131 0.129 0.130
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Acts in coordination with KNL1 to recruit the NDC80 complex to the outer kinetochore.
  • 相關(guān)疾?。?/div>
    Chromosomal aberrations involving CENPK are a cause of acute leukemias. Translocation t(5;11)(q12;q23) with KMT2A/MLL1.
  • 亞細(xì)胞定位：
    Nucleus. Chromosome, centromere. Chromosome, centromere, kinetochore. Note=Localizes exclusively in the centromeres. The CENPA-CAD complex is probably recruited on centromeres by the CENPA-NAC complex.
  • 蛋白家族：
    CENP-K/MCM22 family
  • 組織特異性：
    Detected in several fetal organs with highest levels in fetal liver. In adults, it is weakly expressed in lung and placenta.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 29479

    OMIM: 611502

    KEGG: hsa:64105

    STRING: 9606.ENSP00000242872

    UniGene: Hs.529778