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Human Collagen Type Ⅰ,Col Ⅰ ELISA Kit

  • 中文名稱:
    人Ⅰ型膠原(Col Ⅰ)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E08082h
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人Ⅰ型膠原(Col Ⅰ)酶聯(lián)免疫試劑盒(CSB-E08082h)為雙抗夾心法ELISA試劑盒,定量檢測血清、尿液、組織培養(yǎng)上清液、組織勻漿樣本中的Col Ⅰ含量。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL,適用于纖維化疾病模型研究、骨代謝機制探索以及再生醫(yī)學中膠原沉積的動態(tài)分析。該產(chǎn)品兼容多種實驗場景,包括體外細胞培養(yǎng)體系中膠原分泌的定量評估、動物模型組織修復過程的生物標志物追蹤,以及臨床前研究中Col Ⅰ代謝相關病理生理機制的解析。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    Collagen Type 1,Col 1,_x000D_Collagen Type I,Col I
  • 縮寫:
    Col Ⅰ
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, urine, cell culture supernates, tissue homogenates
  • 檢測范圍:
    1.56 ng/mL-100 ng/mL
  • 靈敏度:
    0.39 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human Col Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 87  
    Range % 80-92  
    1:2 Average % 100  
    Range % 91-110  
    1:4 Average % 105  
    Range % 92-110  
    1:8 Average % 93  
    Range % 86-100  
  • 回收率:
    The recovery of human Col Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 89-99  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    100 2.921 2.985 2.953 2.767  
    50 2.356 2.378 2.367 2.181  
    25 1.826 1.875 1.851 1.665  
    12.5 1.221 1.256 1.239 1.053  
    6.25 0.796 0.800 0.798 0.612  
    3.12 0.512 0.546 0.529 0.343  
    1.56 0.302 0.326 0.314 0.128  
    0 0.188 0.184 0.186    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

引用文獻

產(chǎn)品評價

平均分:
4.7分 - 3 個評價

樣品類型:血清 ,細胞培養(yǎng)上清液

樣品信息:人

稀釋比:其他 1:10

產(chǎn)品評價: human collagen type1 COL1 ELISA試劑盒檢測人血清/血漿,正常、模型和給藥組樣本數(shù)據(jù)很穩(wěn)定,復孔差異較小。血清/血漿結果相近,COL1靈敏度比COL3高,試劑盒穩(wěn)定方便。

By D老師

樣品類型:細胞培養(yǎng)上清液

樣品信息:人

稀釋比:其他 1:5

產(chǎn)品評價: 使用CSB-E08082h試劑盒檢測細胞中collagen 1含量,標曲趨勢正確,數(shù)據(jù)完好。

By 張老師

樣品類型:細胞培養(yǎng)上清液

樣品信息:人

稀釋比:其他 1:20

產(chǎn)品評價: 我用CSB-E08082h產(chǎn)品檢測細胞上清呢膠原蛋白一型,標曲正常。。。產(chǎn)品最后顯色時間延長到30min,OD范圍在0.1-0.3,COLⅠ試劑盒在實驗中,樣本稀釋20-50倍。

By 李老師

靶點詳情

  • 最新研究進展:
    Collagen Ⅰ(Ⅰ型膠原)是一種主要存在于結締組織中的蛋白質,最新研究表明它在疾病發(fā)生和治療中也起著重要作用。例如,肝纖維化和骨質疏松等疾病都與膠原Ⅰ的異常表達有關。此外,一些藥物和生物制劑已經(jīng)用于調節(jié)膠原Ⅰ的合成和代謝,以治療相關疾病。